中文摘要：

以甘蓝ZQ启动抽薹的茎尖为材料,分别提取DNA和RNA,以两对引物扩增并序列拼接得到甘蓝LFYZQ基因DNA序列和cDNA完整编码区,长度分别为2560、1239bp,与花椰菜、拟南芥、芥菜和萝卜同源性分别达到91%、87%、86%、87%。该基因含有3个外显子(452、394、393bp)和2个内含子(514、807bp),共编码412个氨基酸,内含子剪接位点均符合经典GT-AG法则。将LFYZQ与网上公布的12种十字花科植物LFY氨基酸序列按分子进化分成两类:甘蓝LFYZQ与花椰菜以及Jonopsidium属植物Jonopsidium acaule这3个分为一类;其余10种植物分为第二大类。LFYZQ蛋白分子量为46kD,是一个不稳定的疏水蛋白,存在N-十四(烷)酰化位点、蛋白激酶C磷酸化位点、酪蛋白激酶Ⅱ磷酸化位点、酰胺化位点共4种活性位点。

英文摘要：

DNA and RNA were extracted from bolting stem apex of cabbage(Brassica oleracea L.var.capitata L.)cultivar ZQ and LFYZQ gene fragments were cloned by RT-PCR respectively with 2 pairs of primers.LFYZQ of DNA and cDNA sequence fragments assembly were respectively 2 560 bp and 1 239 bp.Analysis of the nucleotide sequence by BLAST on line indicated that homology of LFYZQ was up to 91 % between Brassica oleracea L.var.capitata L.and Brassica oleracea L.var.botrytis L.,up to 87 % between it and Arabidopsis thalian...