中文摘要：

合成了一种水溶性离子液体1-异丁基-3-甲基咪唑四氟硼酸（[i-Bmim]RF4），将其用于呕吐毒素（DON）的酶联免疫检测，结果表明，当体系中[i-Bmim]BF。的浓度为0．014g／mL时，其定量下限为1．41μg／L，比不含该离子液体体系的定量下限4．57斗g／／L提高了约69％；半数抑制浓度为30．20斗∥L；重复测定多次，其半数抑制浓度平均值为29．71μg/L，相对标准偏差为3．26％；用T-2毒素代替DON进行了特异性试验，结果显示，不含离子液体体系中DON与T-2毒素、NIV的交叉反应率相对较高（分别为20．1％、14．9％），而含有0．014g／mL该离子液体体系中DON与T-2毒素、NIV的交叉反应率分别为15．3％、10．2％。方法用于啤酒和麦芽汁中DON加标回收率的测定，实验表明，当体系中[i-Bmim]BF。浓度为0．014g／mL，DON的加标浓度分别为50、70mg／L时，其样品加标回收率均为98％，相对标准偏差分别为2．96％、6．02％。

英文摘要：

An ionic liquid of 1-isobutyl-3-methylimidazole tetrafluoroborate（ [ i-Bmim ] BF4 ） was syn- thesized and applied to the determination of deoxynivalenol（DON） with enzyme-linked immunosorbent assay（ELISA）. A series of experiments were conducted to obtain and compare the detection limits, the half inhibitory concentrations, the reproducibilities and the antiserum specificities of DON with and without 0. 014 g/mL [ i-Bmim] BF4 in the reaction system. Comparative results showed that in the presence of ionic liquid, the detection limit （ 1.41 μg/L ）, the half inhibitory concentration （29.71 μg/L） and the reproducibility（3.26%, n =5） for DON were improved by 69%, 50% and 2%, respectively. The antiserum specificity expressed by cross-reaction rates for DON （ 15.3% ） and T-2 toxin（ 10. 2% ） were improved by about 5%, respectively. The method has been used to deter- mine DON in beer and wort. Comparative results showed that in the presence of 0. 014 g/mL [ i- Bmim ] BF4, the spiked recoveries of DON at two concentration levels of 50, 70 mg/L were both 98%,with relative standard deviations（RSDs） of 2.96%, 6.02%, respectively.