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黄瓜抗枯萎病异源易位植株AT-04的鉴定筛选
  • ISSN号:1000-2030
  • 期刊名称:《南京农业大学学报》
  • 时间:0
  • 分类:S642.2[农业科学—蔬菜学;农业科学—园艺学]
  • 作者机构:[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095
  • 相关基金:国家863计划项目(2004AA241120);国家自然科学基金资助项日(30470120);南京农业大学青年科技创新基金项目(K104009)
中文摘要:

通过对黄瓜种间杂交后代BC1F4群体进行农艺学和细胞学分析,鉴定筛选出抗枯萎病异源易位植株AT-04。农艺学观察表明,其具有小叶、短果、棕色瘤刺、多分枝的特点,形态上偏向原始亲本野生种Cucumis hystrix Chakr.,育性与栽培黄瓜相近。细胞学研究发现,AT-04在有丝分裂的中后期至少有1对染色体的姊妹染色单体分离明显滞后。在减数分裂终变期,平均每个花粉母细胞有0.2个四价体、0.05个六价体和0.05个八价体,约30%的花粉母细胞中存在拉十字的染色体。在中期Ⅰ,7个二价体出现分组分布现象,主要以3+2+2形式存在,表明其至少经历了4次染色体易位。田间调查和人工接种鉴定均表明,易位植株AT-04及其自交后代和野生种C.hystrix对枯萎病具有较高抗性。

英文摘要:

Agronomic and cytological methods were combined in identification and characterization of an alien translocation plant (AT-04) among plants of population BCI F4 that was derived from the interspecific hybridizs, tion between Cucumis savitus L. and C. hystrix Chakr.. In morphology, the plant AT-04 was characterized to have small-sized leaf, short fruit, brown spine and multi-branching habit, which were closely similar to those of the original parent wild species ( C. hystrix), and to have a fertility as high as that of cultivated cucumber. Cytological observation in AT-04 showed that sister chromatids in at least one pair of somatic chromosomes were delayed obviously in separation at late metaphase. At diakinesis, 0. 2, tetravalents, 0. 05 hexavalents and 0. 05 octovalents per pollen mother cell (PMC) were detected, which was absent in cuhivated cucumber. In 30% PMCs, crossed chromosomes occurred at diakinesis. At metaphase Ⅰ , 7 bivalents were frequently detected to separate into three groups, with a form of 3+2+2, which indicated that at least four translocation events once occurred. Results from both field investigation on plants and artificial inoculation on seedlings of AT-04 and its progenies from selfing confirmed their resistance to fusarium wilt as observed in the wild species C. hystrix.

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期刊信息
  • 《南京农业大学学报》
  • 北大核心期刊(2011版)
  • 主管单位:中华人民共和国教育部
  • 主办单位:南京农业大学
  • 主编:郑小波
  • 地址:江苏省南京市卫岗1号
  • 邮编:210095
  • 邮箱:nauxb@njau.edu.cn
  • 电话:025-84395214
  • 国际标准刊号:ISSN:1000-2030
  • 国内统一刊号:ISSN:32-1148/S
  • 邮发代号:28-53
  • 获奖情况:
  • 中国自然科学核心期刊,全国优秀科技期刊,第二届江苏省十佳科技期刊,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:22558