中文摘要：

目的：探讨可溶性Jagged—1／Fc嵌合蛋白（Jagged-1）对重组小鼠粒细胞-巨噬细胞集落刺激因子（rmGM—CSF）和白细胞介素4（rmIL-4）体外诱导小鼠骨髓来源树突状细胞（DC）产生细胞因子的影响。方法：建立rmGM-CSF和rmIL-4体外诱导DC的模型，观察Jagged-1／Fc对DC分化的形态学影响，通过Luminex蛋白液相芯片技术和ELISA检测其细胞因子的表达水平，藉MTT法测定可溶性Jagged-1／Fc诱导的DC对同种异基因淋巴细胞增殖的刺激能力。结果：除了TGF—B，Jag．ged-1／Fc诱导的DC与细菌脂多糖（LPS）或酵母聚糖A诱导的DC不同，表现为生成TNF-α的水平明显降低，IL-4显著增高，而IL-10、IL-6、IL-2、IL-12和IFN-γ的水平与对照组无明显差异。γ分泌酶抑制剂DAPT能逆转Jagged-1／Fc抑制DC生成TNF-仪。混合淋巴细胞反应显示Jagged-1／Fc诱导的DC对T细胞增殖的刺激能力最弱，LPS诱导的DC的刺激能力最强。结论：Jagged-1／Fc诱导的DC倾向介导免疫耐受，指导初始T细胞向Th2细胞偏离。

英文摘要：

AIM： To investigate the in vitro effect of soluble Jagged-1/Fc on the cytokine expression of murine myeloid-derived dendritic cells induced by both rmGM-CSF and rmlL-4. METHODS. The DC culture model under rmGMCSF plus rmlL-4 conditions was established. The influences of Jagged-1/Fc on the morphology of DC was checked, and the cytokine expression were analyzed by LuminexTM system and ELISA. The activation capacity of Jagged-1/Fc-induced DC for allogeneic lymphocytes was detected by MTT assay. RESULTS： Compared with LPS or Zymosan A, the amount of IL-4 expression of Jagged-l/Fc-induced DC increased significantly and the amount of TNF-α expression decreased markedly but α-secretase inhibitor DAPT reversed the inhibition of Jagged-1/Fc on TNF-α expression from DC and returned it to the control level. Jagged-l/Fc altered the a- mount of IL-10, IL-6, IL-2, IL-12 and IFN-γ, expression un- conspicuously and had no effect on TGF-α expression. The mixed lymphocyte reaction （MLR） revealed that Jagged-l/ Fc-induced DC had the weakest ability to activate allogeneic lymphocytes and LPS-induced DC showed the strongest activation ability. CONCLUSION： The results indicate that Jagged-1/Fc-induced DC incline to induce immune tolerance and to prime naive T cells to the Th2 cells.