研究了从某人工湖底泥中筛选出的1株能够降解微囊藻毒素-LR(MC-LR)的菌株JM13的降解特性.实验结果表明,当加入50 mg·L-1淀粉作为外加碳源时,菌株对初始浓度为0.5 mg·L-1MC-LR的降解率可达44.5%;低浓度重金属Cu2+的加入可在一定程度上提高菌株对MC-LR的降解,降解率可达到44.8%;随着时间的延长,菌株对MC-LR的降解率不断增大,到第10天达到52.6%,当投入菌龄为36 h的菌悬液时,降解率可达到55.4%.对该菌株细胞表面疏水性(CSH)的测试结果显示,在菌JM13细胞表面疏水性最大的情况下(即有机∶相水相=3∶4时),添加淀粉及低浓度(0.5 mg·L-1)Cu2+可以在一定程度上抵御降解过程中细菌表面疏水性的降低,使菌体细胞能更好地同污染物接触,从而在一定程度上提高菌株对MC-LR的降解.
A microcystin-LR( MC-LR) degrading strain JM13 was screened out from an artificial lake sediment in Guangzhou,and its ability in the biodegradation of MC-LR was investigated. The experimental results showed that when adding 50 mg·L-1starch as external carbon source,the degradation rate of MC-LR with an initial concentration of 0. 5 mg·L-1reached 44. 5%. The addition of low concentration Cu2+improved the degradation rate of MC-LR to a certain degree,with a degradation efficiency of 44. 8%. The degradation capability increased with time and reached 52.6% on the 10 thday. When a bacterial suspension at cell age of 36 h was added into the system,52. 4% degradation efficiency was achieved. The measurement experiment on cell surface hydrophobicity( CSH) change indicated that the addition of starch and low concentration( 0.5 mg·L-1) Cu2+resisted the decrease of CSH to some extent,making it possible for strain JM13 to better contact the contaminant,thus increasing the degradation of MC-LR.