中文摘要：

目的：分析甲基丙烯酸环氧丙酯（glycidyl methacrylate,GMA）诱导人支气管上皮细胞（16HBE）恶性转化过程中不同时点转化生长因子β（transforming growth factor beta,TGF-β）信号通路的改变,探讨GMA致16HBE细胞恶性转化的分子机制。方法：取经GMA染毒处理转化前期（第10代）、转化后期（第30代）及相应同代龄对照细胞,采用＂人类全基因组表达谱芯片＂分析不同时点转化细胞与同代龄对照细胞之间TGF-β信号通路的改变。结果：TGF-β信号通路在转化前期细胞与同代龄对照细胞之间的表达差异无统计学意义（P〉0.05）,而转化后期细胞与同代龄对照细胞相比表达差异具有统计学意义（P〈0.05）,且发现了11个差异表达的基因。结论：在GMA诱导16HBE细胞恶性转化过程中TGF-β信号通路可能发挥重要作用。

英文摘要：

OBJECTIVE：To analyze the changes of transforming growth factor beta（TGF-β） signal pathway among different stages of malignant transformation of human bronchial epithelial cells（16HBE） induced by glycidyl methacrylate（GMA） and to discuss the molecular mechanisms in the process.METHODS：Cells were harvested at different time points,the 10~（th） generation（protophase） and the 30~（th）（anaphase） generation cells transformed by GMA and the control group in the same generation.Then the changes of TGF-βsignal pathway were analyzed by ＂Human Genome Expression Chip＂ in the process.RESULTS：TGF-βsignaling pathway in cell of the 30~（th） generation showed significant differences between the transformed and control groups,and there were 11 differentially expressed genes.But there were no significant differences in cells of the 10~（th） generation.CONCLUSION：TGF-βsignal pathway possibly plays an important role in the process of malignant transformation of 16HBE induced by GMA.