中文摘要：

针对重要农业害虫大螟，通过序列相似性分析从转录组数据获得2个普通气味结合蛋白（generalodorant-bindingprotein，GOBP）基因cDNA片段，利用RACE技术进一步得到cDNA全长，分别命名为SinfGOBPI和SinfGOBP2。2个基因编码的氨基酸序列和已报道的昆虫同源序列具有较高的相似性，其中sinfGosPl和黄地老虎、小地老虎的相似性最高，分别为91％和90％：SinfGOBP2和谷实夜蛾、甘蓝夜蛾的相似性最高，均为87％。进化分析表明，不同昆虫GOBP被明显分为GOBPl和GOBP2两个亚组，暗示2个亚组GOBP在功能上的分化；此外，相同亚组内GOBP基因问的距离与昆虫间的亲缘关系一致。进一步利用荧光定量PCR，测定了2个基因表达的雌雄差异及时间动态，发现2个基因的表达量在雌雄间均无显著差异；随昆虫日龄的增加，2个GOBP基因的表达量在雌虫中呈上升趋势，但雄虫只在4日龄较高。并讨论了GOBP表达动态与大螟成虫求偶及寻找寄主植物产卵行为的关系。

英文摘要：

Two full-length cDNAs of general odorant-binding protein （GOBP）genes were cloned from Sesamia inferens （Walker）by combination use of informatics and RACE strategy. The genes were named as SinfGOBP1 and SinfGOBP2. The two genes were highly identical in amino acid sequences to the orthologues of other insects,with identities of 91% and 90% for SinfGOBP1 to those in Agrotis segetum and Agrotis ypsilon, and 87% for SinfGOBP2 to those in Helicoverpa zea and Mamestra brassicae. Phylogenetic trees showed that all genes were clustered into two distinct subgroups GOBP1 and GOBP2, suggesting a functional differentiation between them. In addition, the grouping of GOBP genes from different insects well agreed with phylogenetic relations among these insects. Finally, the expression levels of the two SinfGOBP were determined by quantitative real time PCR（qPCR） ,with respect to adults of different sexes and ages. The two genes showed similar expression levels between males and females. With the moth age increasing from 1- to 5-day old, two genes increased in expression levels in females, but in males there was no such a trend. The highest expression occurred in the 4-day old moth in males. This temporal expression pattern was discussed with respect to the adult behaviors of host plant searching in S. inferens.