中文摘要：

目的分析激素性股骨头坏死(SONFH)患者血浆microRNA表达谱变化及生物信息内容,并探讨其意义。方法选取SONFH患者(观察组)和健康志愿者(对照组)各6例,采用Real-time PCR法检测其血浆microRNA相对表达量,筛选差异表达的microRNA。采用Target Scan、mir Base及miRanda三个在线数据库预测交集靶基因,分别进行功能富集性分析和信号转导通路富集(Pathway)分析。结果共检测768个microRNA,两组差异表达的microRNA 16个,其中观察组上调10个,比较明显的是hsa-miR-1207-5p和hsa-miR-887(观察组/对照组>3);下调6个,比较明显的是hsa-miR-96-5p、hsa-miR-576-5p(观察组/对照组<0.5)。三个在线数据库共筛选出交集靶基因887个,其中上调microRNA 483个,hsa-miR-1207-5p未检出交集靶基因,hsa-miR-887检出交集靶基因为GSK3A和CASK;下调microRNA 404个,hsa-miR-96-5p检出交集靶基因114个,hsa-miR-576-5p检出59个。功能富集性分析显示,两组差异表达microRNA调控的靶基因功能主要包括解剖结构形态、间充质细胞增殖、细胞代谢过程等,Pathway分析显示靶基因明显富集于各种信号通路。结论 SONFH患者血浆microRNA明显上调的是hsa-miR-1207-5p和hsa-miR-887,明显下调的是hsa-miR-96-5p、hsa-miR-576-5p,microRNA异常表达可能通过各信号通路参与SONFH的发病。

英文摘要：

Objective To analyze the changes of plasma microRNA expression profile and the content of bioinformat -ics in patients with steroid-induced osteonecrosis of the femoral head ( SONFH ) and to explore its significance . Methods SONFH patients ( observation group ) and healthy volunteers ( control group ) were selected with 6 cases in each group.The real-time PCR was used to detect the relative expression of microRNA in the plasma to screen the differential expression of microRNA .TargetScan , mirBase and miRanda databases were used to predict the target genes which were an -alyzed by gene ontology ( GO) and signal transduction pathway analysis .Results Totally 768 microRNAs were detected by microRNA chip detection .Sixteen microRNAs were different and significant in plasma of SONFH patients .Compared with the control group, 10 were up-regulated (significantly:hsa-miR-1207-5p and hsa-miR-887), and 6 were down-regula-ted (significantly:hsa-miR-96-5p and hsa-miR-576-5p).Totally 887 target genes were screened for the intersection by u-sing TargetScan , mirBase and miRanda three databases .During which , 483 genes were up-regulated , 404 were down-regu-lated.No target genes of hsa-miR-1207-5p were detected, and the target genes of hsa-miR-887 were GSK3A and CASK. Totally 404 microRNAs were down-regulated, 114 Hsa-miR-96-5p-related target genes and 59 hsa-miR-576-5p-related tar-get genes were found .GO and pathway analysis revealed that some differential expression of microRNAs , along with their target genes , were found to be involved in anatomical structure morphogenesis , mesenchymal cell proliferation , positive reg-ulation of metabolic process and significantly enriched in the signal pathway .Conclusions In the plasma of SONFH pa-tients, hsa-miR-1207-5p and hsa-miR-887 were significantly up-regulated, and hsa-miR-96-5p and hsa-miR-576-5p were significantly down-regulated .The abnormal expression of microRNAs in plasma of patients may be involved in the pathogen -esis of SONFH through various signal pathways .