中文摘要：

目的探讨盐酸戊乙奎醚对体外循环大鼠肝脏损伤的影响。方法健康清洁级成年雄性SD大鼠32只，随机分为4组（n=8），假手术组（Sham组）、模型组（Mod组）、盐酸戊乙奎醚低剂量组（L-PHC组）和高剂量组（H-PHC组）。Sham组只置管不转流，Mod组体外循环预充液中不加入盐酸戊乙奎醚，L-PHC组和H-PHC组体外循环预充液中分别加入盐酸戊乙奎醚0．6mg／kg和2mg／kg。于停止体外循环即刻抽取左股动脉血2ml后处死，测定血清谷丙转氨酶（ALT）、谷草转氨酶（AST）活性，观察肝脏组织超微结构改变。结果与Sham组比较，Mod组、L-PHC组、H—PHC组血清ALT、AST活性升高（P〈0．05）；与Mod组比较，L-PHC组、H-PHC组血清ALT、AST活性降低（P〈0．01），病理损伤程度减轻；与L-PHC组比较，H—PHC组血清ALT、AST活性降低（P〈0．05），病理损伤程度减轻。结论盐酸戊乙奎醚0．6mg／kg和2mg／kg可减轻体外循环诱发大鼠肝脏损伤的程度，2mg／kg的效果更明显。

英文摘要：

Objective To investigate the effects of penehyclidine HCl on liver injury induced by cardiopulmonary bypass （CPB） in rats, Methods Thirty-two healthy adult male SD rats weighing 300-450 g were randomly divided into 4 groups （ n = 8 each） group Ⅰ sham operation （S） ; group Ⅱ CPB; group Ⅲ low-dose penehyclidine ＋ CPB （L-PHC） and group iV high-dose penehyclidine ＋ CPB （ H-PHC）. The animals were anesthetized with intraperitoneal 10% chloral hydrate 0,3 ml/100 g, intubated ＂and mechanically ventilated. Femoral artery and right auricle （ via jugular vein） were cannulated. CPB was performed for 60 min. Penehyclidine HCl 0,6 mg/kg and 2 mg/kg were added to the priming solution in group Ill and iV respectively. Blood samples were taken from femoral artery at the termination of CPB for determination of serum alamine aminotransferase （ALT） and aspartate aminotransferase （AST） and the animals were killed. The liver was removed for electron microscopic examination, Results Serum ALT and AST activities were significantly higher in group Ⅱ , Ⅲ and Ⅳ than in group C and were significantly lower in group Ⅲ and Ⅳ than in group Ⅱ , The electron microscopic examination showed that the histologic damage was significantly reduced on group Ⅲ and Ⅳ as compared with group Ⅱ Penehyclidine HCl exerts protective effects against liver injury induced by CPB in a dose-dependent manner,Conclusion Penehyclidine HCl （0.6 and 2.0 mg/kg） can reduce liver injury induced by CPB dosedependently,