中文摘要：

目的：研究复方银黄微型灌肠剂（Yinhuang Micro—enema Compound，YHMCE）对5-脂氧合酶（5-LOX）产物生成的影响，以阐明其抗炎作用的分子机制。方法：体外培养大鼠腹腔巨噬细胞（Peritoneal macrophage，PM），采用高效液相色谱法测定白三烯B4（LTB4）和5-羟二十碳四烯酸（5-HETE）含量变化，以反映5-LOX活性变化。结果：在生药浓度2．24-8．96g／L的范围内，YHMCE呈剂量依赖方式抑制大鼠PMLTB4、5-HETE的生物合成，其IC50分别为含生药2．41g／L与8．43g／L。结论：YHMCE通过抑制5-LOX活性，减少LTB4及5-HETE的产生，可能是其发挥抗炎作用的分子作用机制之一。

英文摘要：

Objective： To study the effect of Yinhuang Micro-enema Compound （YHMCE） on the synthesis of 5-1ipoxygenase products and elucidate its anti-inflammatory mechanisms. Methods： Peritoneal macrophage （PM） obtained from normal rats was cultivated in vitro. The levels of leukotrienes B4 （LTB4） and 5- hydroxy-eicosatetraenoic acid （5-HETE） were detected with reversed phase high-performance liquid chromatography （RP-HPLC） to reflect the activity of 5-1ipoxygenase （5-LOX）. Results： YHMCE between 2.24 g/L and 8.96 g/L could inhibit the synthesis of LTB4 and 5-HETE with dose-dependent manner in rat peritoneal macrophage. The 50 % inhibiting concentration （IC50） was 2.41 g/L and 8.43 g/L ; respectively. Conclusion： The inhibitive effects of YHMCE on the activity of 5-LOX, LTB4 and 5-HETE may account for its anti-inflammatory actions.