东篱科研大数据发现系统（DRDS）

位置：成果数据库 > 期刊 > 期刊详情页

哮喘患儿血清β-防御素-2和免疫球蛋白A、G、M水平及临床意义

ISSN号：1671-332X
期刊名称：《现代医院》
时间：0
分类：R446.62[医药卫生—诊断学;医药卫生—临床医学]
作者机构：[1]广州市越秀区儿童医院,广东广州510115
相关基金：广州市医药卫生科技项目（编号：2009-YB-177）

关键词：哮喘β-防御素-2, 免疫球蛋白A, 免疫球蛋白G, 免疫球蛋白M, 儿童, Asthma children, Human β - defenses 2, Immunoglobulin A, Immunoglobulin G, Immunoglobulin M

中文摘要：

目的探讨β-防御素-2（Human β—defensins2，hBD-2）在哮喘患儿发病中的可能作用。方法对50例5～14岁哮喘急性发作患儿、50例社区获得性肺炎患儿及50例健康儿童分别应用酶联免疫吸附法和免疫比浊法进行血清hBD-2和免疫球蛋白A（Immunoglobulin A，IgA）、免疫球蛋白G（tmmunoglobulin G．IgG）、免疫球蛋白M（Immunoglobulin M，IgM）浓度测定，并分析hBD-2与IgA、IgG、IgM之间的相关性。结果①血清hBD-2浓度，哮喘组为（50．9±16．5）μg／ml，肺炎组为（125．4±19．3）μg/ml，健康对照组为（49．9±15．6）μg／ml，肺炎组高于哮喘组和对照组，差异有统计学意义（P=0．041，P=0．039），哮喘组与对照组比较无统计学差异（P=0．081）。②血清IgA浓度，哮喘组为（1．4±0．7）g／L，肺炎组为（1．4±0．1）μg/L，健康对照组为（1．6±0．8）g／L，哮喘组、肺炎组均低于对照组，差异有统计学意义（P=0．032，P=0．024），哮喘组与肺炎组比较无统计学差异（P=0．090）。③血清IgG浓度，哮喘组为（12±4）g／L，肺炎组为（12±9）g/L，健康对照组为（14±5）g/L，哮喘组、肺炎组均低于对照组，差异有统计学意义（P=0．043，P=0．022），哮喘组与肺炎组比较无统计学差异（P=0．086）。④血清IgM浓度，各组间差异无统计学意义（P〉0．05）⑤血清hBD-2与IgA、IgG、IgM之间不存在线性相关关系（r=0．09，P=0．18；r=0．15，P=0．14；r=0．02，P=0．73）。结论哮喘急性发作患儿血清hBD-2表达水平没有升高，提示机体防御功能存在障碍，这可能是哮喘患儿呼吸道容易发生微生物感染导致急性发作的原因之一。对哮喘患儿同时检测hBD-2、IgA、IgG和IgM能进一步了解机体的免疫功能。

英文摘要：

Objective To investigate the role of human 13 - defenses 2 （ HBD - 2） in the children with asth- ma. Methods Enzyme linked immunoadsorbent assay（ELISA） and immunonephelometry are used among 50 asthma children aged 5 - 14 years in acute stage,50 cases of children with community -acquired pneumonia and 50 healthy children to detect the serum levels of HBD - 2, IgA, IgG and IgM and analyze correlativity between one another. Result suits （1）The serum concentration of HBD - 2 in patients with asthma group, pneumonia group and healthy group was 50. 9 ± 16. 5 μg/ml, 125.4 ± 19. 3 μg/ml and 49. 9 ± 15.6 μg/ml. The serum concentration of HBD -2 in pneumonia group which was compared with asthma group and healthy group was statistically higher （p = 0. 041 ,p = 0. 039）. There was no significant difference between asthma group and healthy group（p = 0. 081 ）.（3）The serum IgA concentration of asthma group （ 1.4 ± 0.7 g/L） and pneumonia group（ 1.4 ± 0. 1 g/L） are both lower than that of control group （ 1.6 ±0. 8 g/L） ,and there are significant difference（p =0. 032,p =0. 024）. There are no significant difference（p = 0. 090） between asthma group and pneumonia group of the serum IgA concentration. （2）Comparing the serum concen- tration of IgG（ 12 ±4 g/L） in asthma group and（ 12 ± 9 g/L） in pneumonia group, there was no significant difference between groups（p = 0. 086）. But they are both lower than that of control group（ 14 ± 5 g/L）, and there are significant differenee（p =0. 043,p =0. 022）. （3）The levels of IgM were similar in all the groups（p 〉0. 05）. （4）There were no linear relationship between serum HBD - 2 and IgA, IgG and IgM （ r = 0. 09 ,p = O. 18 ; r = 0. 15 ,p = 0. 14 ; r = 0.02,p = 0. 73）. Conclusion The serum HBD - 2 expression level of children with acute asthma attacks has not risen, suggesting dysfunction in body defense. It may be one of the reasons why the airways of children with asthma are prone to microbial infection