中文摘要：

选择我国松干蚧分布的代表性地区——浙江金华（ZJJH）、山东青岛（SDQD）、辽宁抚顺（LNFS）采集松干蚧雌成虫,采用优化的RAPD技术和筛选的4条长度为10 bp的随机引物,测定分析3个地理种群松干蚧的遗传多样性及遗传分化.结果表明：从松干蚧单头雌成虫抽提总DNA可以获得足量用于RAPD-PCR反应的模板DNA,雌成虫总DNA分子质量为9 416 bp;在物种水平上,3次重复的种群间分化系数（Gst）分别为0.207 8,0.191 9和0.207 5,表明总的遗传变异中分别有20.78％,19.19％和20.75％的变异存在于种群之间,79.22％,80.81％和79.25％的变异存在于种群内;3次重复中ZJJH与SDQD的Nei＇s遗传距离均为最小,分别是0.040 7,0.035 5和0.044 9,SDQD与LNFS的遗传距离均为最大,分别是0.065 0,0.056 2和0.067 3,表明3个地理种群有着很近的亲缘关系,属于同一虫种;基于Nei＇s遗传距离,利用UPGMA法进行聚类分析的结果表明3个地理种群都属于同一物种,并且在3次重复中ZJJH种群均与SDQD种群先聚为一簇,说明这2个种群间关系较近.

英文摘要：

The Japanese pine bast scale, Matsucoccus matsumurae （Hemiptera： Coccoidea： Matsucoccidae）is an important invasive pest in pine forests of China. However, the classification and the population genetic differentiation of this species over various distribution regions have been a problem in the pest management decision-making. In order to provide molecular evidence for the species identification and scientific management of the pest, we collected samples from Fushun in Liaoning Province（ LNFS）, Qingdao in Shandong Province （SDQD）and Jinhua in Zhejiang Province （ZJJH） , respectively, and applied RAPD-PCR technigue with 4 random primers to investigate the genetic variation of the three geographic populations. The results showed that the DNA extracted from individual female adult was enough for RAPD- PCR. The genome size of the species was 9 416 bp. At species level, coefficient of genetic differentiation （G~,） of the population with three replicates was 0. 207 8, 0. 191 9 and 0. 207 5, respectively, indicating that there were 20.78% , 19.19% and 20.75% of genetic diversity resided among populations, and 79.22% , 80.81% and 79.25% of genetic diversity within populations. The minimum genetic distance derived from the three replicates was 0. 040 7, 0. 035 5 and 0. 044 9 between ZJJH and SDQD, respectively, and the maximum ones was 0. 065 0, O. 056 2 and O. 067 3 between SDQD and LNFS, which suggested that the three geographic populations would be very close in genetic relationship and that belong to the same species. According to the UPGMA dendrograms, the 3 populations could be grouped into two clades and one contained ZJJH and SDQD and the other contained LNFS only, which indicated a closer genetic relationship between the ZJJH and SDQD populations. This research provides a scientific basis for the pest management and has an importantsignificance in using sex pheromones and the natural enemies of the pine bast scale for biological control in the future.