中文摘要：

目的探讨大鼠骨髓间充质干细胞（rat bone-derived mesenchymal stem cells,r BMSCs）膀胱壁注射法改善大鼠逼尿肌无力（acontractile detrusor,ACD）的可能性。方法 40只SD雌性大鼠分为4组,每组10只：正常大鼠对照组、低温诱导ACD组、生理盐水治疗组、r BMSCs治疗组。常规分离培养r BMSCs至第3代。建立大鼠膀胱壁冻伤模型模拟逼尿肌无力的情况,将r BMSCs或生理盐水注入大鼠膀胱壁肌层,14 d后检测各组大鼠膀胱排尿功能,逼尿肌肌条收缩力,RT-PCR和Western blot检测平滑肌收缩相关蛋白（α-SMA、Calponin和SM-MHC）的表达情况,HE染色了解各组大鼠膀胱壁组织学改变情况。结果 r BMSCs治疗组膀胱内压、肌条收缩力显著高于低温诱导ACD组和生理盐水治疗组（P〈0.01）,恢复超过50%。治疗组α-SMA、Calponin及SM-MHC的表达明显高于低温诱导ACD组和生理盐水治疗组（P〈0.01）。结论 r BMSCs通过膀胱壁注射能有效改善大鼠逼尿肌无力。

英文摘要：

Objective To explore the possibility of alleviating acontractile detrusor （ACD） by cytotherapy with rat bone marrow-derived mesenchymal stem cells （rBMSCs） injected into the bladder wall. Methods Forty Sprague-Dawley female rats were divided into 4 groups： a control group （ G1, n = 10） , a low-temperature modeling group （ G2, n = 10 ） , a normal saline treatment group （ G3, n = 10 ）, and a rBMSCs treatment group （G4, n = 10）. The rBMSCs were isolated and cultured as reported before. ACD was induced via cryoinjury of the bladder, and rBMSCs were directly injected into the bladder wall. After 14 d, the bladder micturition function, bladder detrusor strip tension, expressions of smooth muscle contraction- related markers （ct-SMA, Calponin and SM-MHC ） and histological changes were assessed in each group. Results Western blotting and RT-PCR results indicated that compared with the G2 and G3 groups, the mRNA and protein levels of ct-SMA, Calponin and SM-MHC were markedly increased in the G4 group （ P 〈 0. 01 ）. Cystometry and muscle strip intension test demonstrated the detrusor contractibility was improved in the G4 group, and the detrusor function was recovered by over 50%. Conclusion The injection of rBMSCs into the bladder wall can alleviate ACD in the rat model.