以黄嘴白鹭为研究对象,探讨并建立其遗传多样性分析的Psil/Mse I AFLP分子标记方法.采用黄嘴白鹭13个样品作为一个种群的池DNA,通过对AFLP方法中DNA提取、酶切、连接、预扩增、选择性扩增、电泳和银染等条件进行优化,建立了一套适合黄嘴白鹭的AFLP技术优化体系,为黄嘴白鹭遗传多样性的分析奠定研究基础.
An AFLP technique for the genetic diversity analysis ofEgretta eulophotes was established in this study. 13 genomic DNA samples of Egretta eulophotes were taken as a population DNA pool. The conditions of genomic DNA extraction, enzyme digestion, ligation, preamplification, selective amplification, electrophoresis and silver staining were optimized in the Pst I/Mse I AFLP analysis. The AFLP technique established in this study was applicable for the genetic diversity analysis of Egretta eulophotes in the future.