中文摘要：

目的研究白细胞介素-8（IL8）对血管内皮细胞紧密连接的影响。方法采用免疫荧光染色检测经不同浓度和时间IL-8处理后EA．hy926细胞的3种紧密连接蛋白occludin、claudin-5和ZO一1的形态和分布；逆转录PCR（RT-PCR）检测3种蛋白mRNA的表达水平。结果IL-8可改变内皮细胞紧密连接蛋白occludin、claudin．5和ZO-1的分布和表达强度；随着IL-8浓度和作用时间的增加，occludin、claudin-5和ZO-1mRNA表达逐渐降低。结论IL-8改变occludin、claudin-5和ZO-13种紧密连接蛋白的分布和表达，并下调其mRNA表达，且呈现出一定的时间和浓度依赖关系。

英文摘要：

cells. Methods Objective To investigate the influence of IL-8 on the tight junction of vascular endothelial was used to observe the modality and the distribution of three tight junction proteins （occludin,claudin-5 and ZO-1） of the EA.hy926 cells treated with IL-8 under different concentrations and different times. RT-PCR was used to measure the mRNA expression of these three proteins. Results The results demonstrated that IL-8 could change the distribution of occludin, claudin-5 and ZO-1 in EA.hy926 cells, and the mRNA expression of occludin, claudin-5 and ZO-1 decreased with the increase of IL-8 concentration and treated time. Conclusion The effects of IL-8 on the distribution and the expression of occludin, claudin-5 and ZO-1 are dose and time-dependent.