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大别山山核桃种群遗传多样性研究
  • ISSN号:1005-264/X
  • 期刊名称:《植物生态学报》
  • 时间:0
  • 分类:S965.1[农业科学—水产养殖;农业科学—水产科学] S664.1[农业科学—果树学;农业科学—园艺学]
  • 作者机构:[1]北京林业大学生物科学与技术学院,北京100083, [2]浙江林学院现代森林培育省级重点实验室,浙江临安311300, [3]淮北煤碳师范学院生物系,安徽淮北235000
  • 相关基金:国家自然科学基金(30371124)、浙江省科技厅重大招标项目(0211025)、浙江省自然科学基金重大项目(ZA0208)和杭州市科技局重大攻关项目(2002112A12)
中文摘要:

为了更有效地保护和合理开发大别山山核桃(Carya dabieshanensis)资源,该文利用RAPD分子标记技术,对3个天然大别山山核桃种群的90个单株的遗传多样性、种群内和种群间的遗传变异进行了研究,结果表明:20对10bp随机引物共检测到238条谱带,其中多态带为162条,占68.1%。遗传多样性分析结果显示:Shannon多样性指数为0.476l,58.18%的变异分布于群体内,而种群间变异占了41.82%;Nei指数群体总基因多样度为0.3145,群体内平均基因多样度(ns)为0.1865,群体间的基因多样度(HST)为0.1280,群体,vei基因分化系数(Gsr)为0.4067,说明40.67%的变异存在于种群间,群体内的变异占了总变异的59.33%,与Shannon多样性指数相比基本一致,均表明种群内有较丰富的遗传变异,这为优良品种选育提供广阔前景;种群间的基因流(Nm)为0.7306。证明种群间遗传交换较小。这与环境适应性和高山阻隔有一定的关系。

英文摘要:

The genetic diversity and genetic variation within and among three natural Carya dabieshanensis populations were studied using RAPD analysis. The result showed that 238 loci were detected by using 20 random primers (10 bp) of which 162 loci were polymorphic (68.1%). Species genetic diversity indicated by Shannon' s index was 0.476 1, 58.18 % of which was due to within population genetic diversity and 41.82 % among population variation. Species gene diversity indicated by Nei's index was 0.314 5, gene diversity within populations (Hs) was 0. 186 5, gene diversity among populations (HST) was 0. 128 0 and the coefficient of gene differentiation (GST) among the populations was 0.406 7, 59.33% of which was within populations and 40.67 % was among populations. The gene diversity estimated by Nei' s index was consistent with that estimated by Shannon' s index. These results suggest that there is rich genetic within population variation, which offers excellent prospects for seeding selection. The estimate of gene flow from GsT(Nm) was 0.730 6, indicating that genetic recombination between populations is quite small, which probably is related to environmental adaptations of C. debieshanensis and population isolation in their high mountain habitat.

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期刊信息
  • 《植物生态学报》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国科学院植物研究所 中国植物学会
  • 主编:方精云
  • 地址:北京香山南辛村20号
  • 邮编:100093
  • 邮箱:apes@ibcas.ac.cn
  • 电话:010-62836134
  • 国际标准刊号:ISSN:1005-264/X
  • 国内统一刊号:ISSN:11-3397/Q
  • 邮发代号:82-5
  • 获奖情况:
  • 中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国生物科学数据库,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),瑞典开放获取期刊指南,中国北大核心期刊(2000版)
  • 被引量:37395