中文摘要：

以皱纹盘鲍幼鲍Haliotis discus hannaiIno（平均壳长：13.90±0.01mm;平均体重：482.2±2.2mg）为研究对象,在室内循环水养殖系统中进行112d的摄食生长实验,以海带为对照,探讨饲料中不同叶酸含量（0.06、1.44、2.86、5.88、14.27、35.71mg/kg饲料）对皱纹盘鲍脂肪酸组成的影响。实验结果表明,（1）叶酸缺乏可显著降低皱纹盘鲍C20∶4n-6和C20∶5n-3的含量（P〈0.05）,却显著提高C18∶3n-3和C22∶6n-3的含量（P〈0.05）;（2）海带与实验基础饲料中的脂肪酸组成存在显著差异,海带中的C14∶1、C16∶0、C18∶1n-9、C18∶3n-3、C20∶4n-6、C20∶5n-3含量显著高于饲料组（P〈0.05）,而C18∶0、C18∶1n-7、C18∶2n-6、C20∶0、C22∶6n-3均显著低于饲料组（P〈0.05）;（3）海带组鲍鱼的C20∶4n-6、C20∶5n-3显著高于饲料组鲍鱼（P〈0.05）,而C18∶1n-9、C18∶1n-7、C18∶2n-6、C22∶6n-3则显著低于饲料组鲍鱼（P〈0.05）。（4）C16∶0、C16∶1、C18∶0在鲍鱼体内的含量比较稳定,不受饲料中叶酸含量和脂肪酸组成的显著影响（P〉0.05）。这些结果说明,饲料中叶酸缺乏导致皱纹盘鲍脂肪酸组成发生改变,很可能抑制C18∶2n-6和C18∶3n-3的碳链延长和去饱和作用,从而减少C20∶4n-6和C20∶5n-3的合成。此外,饲料中的脂肪酸组成也是影响皱纹盘鲍体组织脂肪酸组成的一个重要因素。

英文摘要：

In this experiment, the effects of folic acid in diets on fatty acids composition in abalone （Haliotis discus hannai Ino） were studied. The experiment lasted 16 weeks, with the initial mean weight and mean shell length of abalone being 482.2 ± 2.2 mg and 13. 90 ±0.01 mm, respectively. Different levels of folic acid content in formulated diets were set up, viz. 0.0, 1.4, 2.8, 5.6, 14.0, and 35.0 mg/kg. Kelp was used as the control diet. The main results were as follows. （1） Folic acid deficiency could make abalone significantly lower its C20 ：4n-6 and C20 ： 5n-3 contents, and enhance its C18 ： 3n-3 and C22 ： 6n-3 contents （P〈0.05）. （2） There existed significant differences in fatty acids composition between kelp and formulated diet. The contents of C14 ： 1, C16 ： 0, C18 ： ln-9, C18 ： 3n-3, C20 ： 4n-6, and C20 ： 5n-3 in kelp were significantly higher than those in formulated diet（P〈0.05）, whereas the contents of C18 ： 0, C18 ： 1n-7, C18 ： 2n-6, C20 ： 0, and C22 ： 6n-3 in kelp were significantly lower than those in formulated diet（P〈0.05）. （3） Abalone fed with kelp were significantly higher than that fed with formulated diet in contents of C20 ： 4n-6 and C20 ： 5n-3（P〈0.05）, whereas abalone fed with kelp were significantly lower than that fed with formulated diet in contents of C18 ：1n-9, C18：1n-7, C18 ： 2n-6, and C22 ： 6n-3（P〈0.05）. （4） The contents of C16 ： 0, C16 ： 1 and C18 ： 0 in abalone were relatively stable, not being affected by folic acid in diets easily. These results suggested that folic acid deficiency in diet could lead to the changes of fatty acid composition in abalone, and thereby suppress the carbon chain elongation and desaturation of C18：2n-6 and C18 ： 3n-3, lessening the synthesis of C20 ： 4n-6 and C20 ： 5n-3. Furthermore, the fatty acid composition in diets was also an important factor affecting the fatty acid composition in abalone.