中文摘要：

目的利用神经母细胞瘤（NB）临床标本筛选与恶性进展相关的基因。方法利用比较基因组杂交技术和表达谱基因芯片技术筛选人类17号染色体上某些区域可能存在的与NB密切相关的候选基因,并结合生物信息学分析和RT-PCR检测结果对候选基因进行进一步鉴定。结果通过初步筛选,在人类17号染色体增添区域发现了与NB恶性进展相关的新基因（ncRAN）,该基因包含2个剪切体,Nbla10727和Nbla12061。ncRAN在各组织中及MYCN单/多拷贝组细胞中呈现差异性表达,其高表达与不良预后密切相关（P=0.000 221）。结论在17号染色体增添区所鉴定的新基因很可能是1个与NB恶性进展密切相关的癌基因。

英文摘要：

Objective To discover the molecular mechanisms underlying progression of human neuroblastoma by screening the candidate genes in clinical specimens.Methods The candidate genes mapped on human chromosome 17q gain,which was significantly associated with the neuroblastoma,were screened by combining the array-CGH results with expression profiling,and identified by bioinformatic analysis and RT-PCR.Results A novel gene linked to progression of neuroblastoma named ncRAN was found on chromosome 17q gain,which included two transcripts,Nbla10727 and Nbla12061,and differential expression can be detected in various tissues and neuroblastoma cell lines with a single copy and amplification of MYCN.Furthermore,high expression of ncRAN was associated with poor prognosis（P =0.000 221）.Conclusion The novel gene mapped on chromosome 17q gain may be an oncogene which is associated with progression of neuroblastoma.