中文摘要：

HSP90是重要抗逆分子,为了探讨近江牡蛎CrassostreahongkongensisHSP90分子在抗逆中的作用,本研究建立实时荧光定量PCR方法,研究了在孔雀石绿胁迫下近江牡蛎HSP90基因在外套膜、消化腺、鳃、闭壳肌4种器官组织中的表达变化规律。结果显示,在浓度1μg/L的孔雀石绿处理下,4种器官组织中的HSP90基因表达量均出现显著性高表达,且在外套膜、消化腺和闭壳肌3种器官组织中的表达规律为随时间推移呈现先升高后下降的趋势,表明近江牡蛎HSP90在抗孔雀石绿胁迫中可能起重要作用。

英文摘要：

Heat shock protein 90（HSP90） is a highly conserved molecular chaperone that plays key roles in the folding,and maintenance of structural integrity of proteins.In order to investigate the HSP90＇s effect of the oyster Crassostrea hongkongensis against stress factors,we established a real-time fluorescent quantitative RT-PCR method to examine the transcript expression patterns of HSP90 mRNA in mantle,digestive gland,gills,and muscle of the oyster exposed to malachite green（MG） solution.The results showed that HSP90 mRNA expression in mantle and digestive gland was up-regulated gradually at first,and peaked at 4 days and 1 day,then gradually decreased to the control level at 12 days and 2 days,respectively,when the oysters were treated in MG solution（1 μg/L）.The phenomenon was defined as time-dependent expression pattern of HSP90 gene.However,the HSP90 mRNA levels in muscle and gills were significantly increased on day 8 and 12,respectively,but both of them did not drop to the control level during the period of MG exposure.The fact of up-regulation of HSP90 mRNA expression induced by MG solution treatment suggests that HSP90 gene of the oyster may play an important role in resistance of stress factors,like MG.