中文摘要：

目的 利用小肠黏膜下层（SIS）的免疫隔离作用包被大鼠胰岛和间充质干细胞（MSC）构建人工生物胰岛,观察该胰岛移植对糖尿病大鼠的治疗作用.方法 分离、纯化大鼠MSC和胰岛后,分为以下3组：单纯胰岛培养组（a组）、SIS与胰岛培养组（b组）、SIS包被MSC与胰岛共培养组（c组）.采用葡萄糖刺激胰岛素分泌实验检测各组胰岛的功能,采用免疫荧光检测胰岛的CD31表达情况,采用酶联免疫吸附试验测定各组上清液中表皮细胞生长因子和血管内皮生长因子-A表达.成功构建大鼠糖尿病模型,将糖尿病大鼠分为A组、B组和C组,分别对应的应用a组、b组和c组胰岛进行胰岛移植,监测各组大鼠血糖水平的变化及移植物存活情况.结果 体外实验中,c组胰岛素分泌功能明显升高（P＜0.01）,且CD31的表达高于其他两组,表明MSC对胰岛功能及血管形成具有保护作用.体内实验中,各组血糖均有所下降,但C组大鼠的血糖水平最佳,且移植物存活时间从（22.8＆#177;4.12）d提高到（57.6＆#177;2.84）d.结论 猪小肠黏膜下层包被大鼠MSC可提高大鼠胰岛的功能,促进胰岛移植物的血管生成,对糖尿病大鼠具有良好的治疗作用.

英文摘要：

Objective In the present study,we constructed artificial pancreas using small intestinal submucosa （SIS） coated mesenchymal stem cells （MSCs） and islets to observe the effect on diabetic rats.Method The experiment was divided into three groups：A,islets;B,SIS-islets;C,SIS-MSCs-islets.Islet function was assessed by measuring glucose-stimulated insulin secretion in vitro.Cytokines were detected in culture supernatants by ELISA and CD31 determined by immunohistochemistry.In vivo,graft function and survival were monitored by blood glucose levels.Result In vitro,the stimulation index of group C was significantly higher,and CD31 protein expression secretion was greater than other groups.These results suggest that MSCs enhance islet function and revascularization.In vivo,blood glucoses in three groups were decreased,and the islet graft survival time in group C was prolonged significantly from 22.8 ＆#177; 4.12 to 57.6 ＆#177; 2.84 days.Conclusion Our results have suggested that MSCs enhanced revascularization and improved the efficacy of coated islets in SIS.The artificial pancreas may offer a new approach for curing diabetes.