中文摘要：

目的：探讨脊髓神经元限制性沉默因子（NRSF ）在瑞芬太尼诱发切口痛小鼠痛觉过敏中的作用。方法健康清洁级成年雄性昆明小鼠56只，体重20～25 g ，采用随机数字表法分为7组（ n＝8）：对照组（C组）、切口痛组（I组）、切口痛＋瑞芬太尼组（IR组）、NRSF反义寡核苷酸组（NAS组）、切口痛＋NRSF反义寡核苷酸组（I＋NAS组）、切口痛＋瑞芬太尼＋NRSF错义寡核苷酸（IR＋NMS组）和切口痛＋瑞芬太尼＋NRSF反义寡核苷酸（IR＋NAS组）。C组、I组和IR组鞘内注射人工脑脊液5μl ，NAS组、I＋NAS组和IR＋NAS组鞘内注射NRSF反义寡核苷酸10μg ，IR＋ NMS组鞘内注射NRSF错义寡核苷酸10μg ，均为1次/d ，连续3 d。于末次鞘内注射后30 min时，C组和NAS组皮下输注生理盐水0.4 ml ，I组和I＋NAS组制备切口痛模型，同时皮下输注生理盐水0.4 ml ，IR组、IR＋NMS组和IR＋NAS组制备切口痛模型，同时皮下输注瑞芬太尼0.04 mg/kg。于术前3 d （T0）、4 h （T1）、术后4 h（T2）、12 h（T3）、24 h（T4）和48 h（T5）时测定机械缩足阈（PWMT）和热缩足阈（PWTL）。结果与C组比较，I组、IR组、I＋NAS组、IR＋NMS组和IR＋NAS组T2-5时PWMT和PWTL降低（ P＜0.05），NAS组各时点PWMT和PWTL差异无统计学意义（ P＞0.05）；与I组比较，IR组和IR＋NMS组T2-5时PWMT和PWTL降低（ P＜0.05），I＋NAS组各时点PWMT和PWTL差异无统计学意义（ P＞0.05）；与IR组比较， IR＋NMS组各时点PWMT和PWTL差异无统计学意义（ P＞0.05），IR＋NAS组T2-5时PWMT和PWTL升高（ P＜0.05）。结论脊髓NRSF参与了瑞芬太尼诱发切口痛小鼠痛觉过敏的形成和维持。

英文摘要：

Objective To evaluate the role of neuron-restrictive silencer factor （NRSF） in the spinal cord in remifentanil-induced hyperalgesia in a mouse model of incisional pain （IP） .Methods Fifty-six male Kunming mice were randomly divided into 7 groups （n=8 each）：control group （group C） ,IP group （group I） ,IP ＋remifentanil group （group IR ） , NRSF antisense oligonucleotide group （NAS group ） , IP ＋ NRSF antisense oligonucleotide group （I＋NAS group ） ,IP ＋ remifentanil ＋ NRSF mismatch oligonucleotide group （IR＋NMS group） , and IP ＋ remifentanil ＋ NRSF antisense oligonucleotide group （IR ＋ NAS group ） . Artificial cerebrospinal fluid 5 μl was injected intrathecally once a day for 3 consecutive days in C ,I and IR groups .NRSF antisense oligonucleotide NAS 10μg was injected intrathecally once a day for 3 consecutive days in NAS ,I＋NAS and IR ＋ NAS groups . NRSF mismatch oligonucleotide 10 μg was injected intrathecally once a day for 3 consecutive days in IR＋NMS group .A 1-cm longitudinal incision was made through skin ,fascia and muscle of the plantar aspect of the right hindpaw to establish the model of incisional pain in sevoflurane-anesthetized rats .At 30 min after the last injection ,normal saline 0.4 ml was infused subcutaneously in C and NAS groups ,the model＆nbsp;was established and normal saline 0.4 ml was subcutaneously infused simultaneously in I and I＋NAS groups ,and the model was established and remifentanil 0.04 mg/kg was subcutaneously infused simultaneously in IR ,IR＋NMS and IR＋NAS groups .At 3 days before operation （T0 ） ,4 h before operation （T1 ） and 4 ,12 ,24 and 48 h after operation （T1-5 ） ,mechanical paw withdrawal threshold to von Frey stimuli （PMWT ） and paw withdrawal latency to thermal nociceptive stimulus （PTWL ） were measured .Results Compared with C group ,the PWMT and PWTL were significantly decreased at T2-5 in I ,IR ,I＋NAS ,IR＋NMS and IR＋NAS groups （ P0.05 ） .Compared with I group ,th