应用组织学、免疫组织化学和生物统计分析相结合的方法,对黄胫小车蝗(Oedaleus infernalis Sauss.)配子发生期间原癌基因c—kit的特异表达进行了研究.结果表明:精子发生过程中,精原细胞、初级精母细胞、次级精母细胞和成熟精子中均有不同程度的c—kit蛋白表达,精巢末端还有较粗大的阳性颗粒分布;卵子发生过程中,第1~6阶段卵母细胞中有不同程度的c—kit蛋白特异性表达,但随着卵黄的发生逐渐消失.
Abstract:By using histology, imlnunohistochemistry and biostatistics, the c-kit special expression in those phases were studied by collecting Oedaleus infernalis Sauss. from the Siping area,Jilin province, China. The results showed that: (1) In spermatogenesis, there were different expression levels of c-kit protein in spermatogonia, primary spermatocytes, second spermatocytes and mature sperms, and the biggest positive granules were found at the end of spermary. (2)In oogenesis, there were different expression levels of c-kit protein from the 1st to 6th stage of oocytes but the positive granules gradually disappeared with the following vitellogenesis.