Bioleaching 是一种环境友好、节俭的技术把重金属从污染土壤移开。这个工作的目的是发现土生土长的紧张补救 Zn, Pb, Cu 和 Cd 污染的土壤。从熔炼工业的一个本地人的土壤被选择的紧张被发现能生产许多器官的酸并且降级液体媒介的 pH 价值。真菌紧张作为毛丛 Chrysogenum 被识别(P。Chrysogenum ) 由定序 18srDNA 并且它的。用 P 的 Bioleaching 状况。Chrysogenum 被优化。葡萄糖是为 P 的最好的碳来源。Chrysogenum 和无机的氮比器官的氮好。另外,中立答案和房间温度为 P 是合适的。Chrysogenum 到 bioleach。在一步舞 bioleaching, bioleaching 比率为 Zn 是 39.95% , 9.4% 为 Pb, 34.89% 为 Cu 并且 49.59% 为 Cd,为 Zn 是 53.89% , 14.44% 为 Pb, 55.53% 为 Cu 并且 62.81% 为在二拍子的圆舞 bioleaching 的 Cd。二拍子的圆舞 bioleaching 的效率比一步舞 bioleaching 好。P。Chrysogenum 在把重金属从污染土壤移开是有效的。
Bioleaching is an environment-friendly and economical technique to remove heavy metals from contaminated soil. The objective of this work is to find out an indigenous strain to remedy soil contaminated by Zn, Pb, Cu and Cd. A strain which was selected from the soil of a local smelting industry was found to be able to produce many organic acids and degrade pH value of the liquid medium. The fungus strain is identified as Penicillium Chrysogenum (P Chrysogenum) by sequencing 18srDNA and ITS. Bioleaching condition using P Chrysogenum is optimized. Glucose is the best carbon source for P Chrysogenum and inorganic nitrogen is better than organic nitrogen. In addition, neutral solution and room temperature are fit for P Chrysogenum to bioleach. In the one-step bioleaching, the bioleaching ratios are 39.95% for Zn, 9.4% for Pb, 34.89% for Cu and 49.59% for Cd, which are 53.89% for Zn, 14.44% for Pb, 55.53% for Cu and 62.81% for Cd in the two-step bioleaching. The efficiency of two-step bioleaching is better than the one-step bioleaching. P Chrysogenum is effective in removing heavy metals from the contaminated soil.