对三叶草接种AM真菌根内球囊霉,用盆栽试验和分根试验测定根系的菌根侵染率和抗氧化酶活性,研究AM真菌对根系抗氧化酶活性的影响以及该影响的系统性。结果表明,盆栽试验中接种根内球囊霉显著提高了根系中SOD、POD、CAT的活性,表明AM真菌可以促进根系的抗氧化酶活性;分根试验中一半根系接种了根内球囊霉的植株,其另一半未接种的根系SOD、POD活性也增加,表明AM真菌对根系抗氧化酶系统的促进具有系统效应。由于抗氧化酶系统是植物产生抗逆性的生理生化基础,可以推测,AM真菌对根系抗氧化酶活性的系统性提高有助于保护根系整体,而非仅仅保护受侵染根段。
Clover plants(Trifolium repens L.) were inoculated with arbuscular mycorrhizal(AM) fun-gus,Glomus intraradices,in pot and split-root experiments.The mycorrhizal colonization and antioxi-dase activities in roots were measured for the influence of Glomus intraradices on the root antioxidase activities and the systematization of the influence.Results indicated that G.intraradices significantly enhanced the activities of SOD,POD,CAT in roots in the pot experiment,suggesting an increased an-tioxidase activity by AM fungus.For the plants with half roots inoculated with G.intraradices in the split-root experiment,the activities of SOD,POD also increased in the other half roots without G.intra-radices,suggesting the systematization of the increase in antioxidase by AM fungus.Given that the anti-oxidase system is the physilogical and biochemical basis of the resistance to diverse stresses in plants,this systematical increase may contribute to the protection of the entire roots,not of the infected ones only.