中文摘要：

目的：测定大鼠体内咖啡因、氨苯砜和氯唑沙宗的血药浓度,计算药动学参数。方法：取大鼠6只,ig咖啡因、氨苯砜和氯唑沙宗混合溶液,给药剂量分别为1.5、2、3 mg/kg,于给药前及给药后0.5、1、2、3、4、6、8、12、24 h取血0.2-0.3 ml;血浆样品用固相萃取法处理,采用高效液相色谱法,以N-（2-羟乙基）邻苯二甲酰亚胺为内标,测定咖啡因、氨苯砜和氯唑沙宗的血药浓度;并用DAS 2.0软件计算药动学参数。结果：咖啡因、氨苯砜及氯唑沙宗检测质量浓度的线性范围均为0.2-30μg/ml（r分别为0.996 4、0.996 1、0.998 8）,定量限均为0.2μg/ml,低、中、高质量浓度水平的方法回收率分别为（84.8±3.6）%-（111.4±10.2）%（RSD为4.3%-9.8%）、（107.0±13.3）%-（113.5±8.1）%（RSD为7.1%-14.0%）、（104.2±10.8）%-（111.1±12.2）%（RSD为8.0%-11.0%）（n=3）;药动学参数tmax为（1.70±0.99）、（1.50±1.00）、（1.92±0.80）h,t1/2为（0.73±0.22）、（2.77±1.35）、（2.78±2.34）h,cmax为（2.60±0.50）、（5.78±1.19）、（9.76±1.37）mg/L,AUC0-t为（8.43±0.79）、（20.68±1.91）、（26.71±2.45）mg·h/L（n=6）。结论：本法操作简便、灵敏度高、结果准确,可用于咖啡因、氨苯砜和氯唑沙宗的血药浓度测定和药动学研究。

英文摘要：

OBJECTIVE：To determine plasma concentration of caffeine,dapsone and chlorzoxazone in rats,and to calculate pharmacokinetic parameters. METHODS：6 rats were given the mixture of caffeine,dapsone and chlorzoxazone intragastrically,1.5,2 and 3 mg/kg,respectively. 0.2-0.3 ml blood were collected before medication and 0.5,1,2,3,4,6,8,12,24 h after medication.The plasma sample was treated with solid phase extraction. The plasma concentration of caffeine,dapsone and chlorzoxazone were determined by HPLC using N-（2-Hydroxyethyl）phthalimide as internal standard. The pharmacokinetic parameters were calculated using DAS 2.0 software. RESULTS：The linear ranges of caffeine,dapsone and chlorzoxazone were all 0.2-30 μ g/ml（r were 0.996 4,0.996 1,0.998 8,respectively）. The limit of quantitation were 0.2 μg/ml. The recoveries of low-concentration,medium-concentration and high concentration were（84.8±3.6）%-（111.4±10.2）%（RSD were 4.3%-9.8%,n=3）,（107.0±13.3）%-（113.5±8.1）%（RSD were 7.1%-14.0%,n=3）,（104.2±10.8）%-（111.1±12.2）%（RSD were 8.0%-11.0%,n=3）. Pharmacokinetic parameters were as follows as tmax（1.70±0.99）,（1.50±1.00）,（1.92±0.80）h;t1/2（0.73±0.22）,（2.77±1.35）,（2.78±2.34）h;cmax（2.60±0.50）,（5.78±1.19）,（9.76±1.37）mg/L;AUC0-t（8.43±0.79）,（20.68±1.91）,（26.71±2.45）mg·h /L（n=6）.CONCLUSIONS：The method is simple,sensitive and accurate,and can be used for the plasma concentration determination and pharmacokinetic study of caffeine,dapson and chlorzoxazone.