中文摘要：

目的研究力生长因子（mechano-growth factor,MGF）E肽对前交叉韧带（anterior cruciate ligament,ACL）成纤维细胞活力、迁移与侵袭的影响。方法以ACL成纤维细胞为研究对象：（1）经不同浓度（0、10和100μg/L）MGF E肽处理细胞24 h后,去除培养基,换成1%血清浓度DMEM低糖培养基培养6和30 h后,检测细胞活力、DNA含量、细胞凋亡、基质金属蛋白酶-2（matrix metalloproteinases-2,MMP-2）和基质金属蛋白酶-9（MMP-9）活性变化及I型胶原（COL I）、III型胶原（COL III）mRNA表达;（2）使用不同浓度（0、5、10、20、50和100μg/L）MGF E肽处理ACL成纤维细胞48 h后,检测细胞活力与MMP-2活性,并利用划痕实验与Transwell实验分别检测细胞迁移与侵袭能力。结果 （1）6 h时,10μg/L浓度MGF E肽显著促进MMP-2、MMP-9活性,对细胞活力、增殖、凋亡及COL I、COL III mRNA表达无影响。100μg/L浓度MGF E肽显著促进MMP-2、MMP-9活性,并提高了COL I、COL III mRNA表达,但对细胞活力、增殖、凋亡无影响。30 h时,10μg/L浓度MGF E肽显著促进MMP-9活性及COL I、COL III mRNA表达,对细胞活力、增殖、MMP-2活性及细胞凋亡无影响。100μg/L浓度MGF E肽显著促进MMP-9活性及COL III mRNA表达,但对细胞活力、增殖、MMP-2活性、细胞凋亡、COL I mRNA表达无显著性调控作用。（2）MGF E肽显著促进ACL成纤维细胞的迁移与侵袭,且呈一定程度浓度依赖性;迁移与侵袭过程可能依赖于MMP-2活性。结论 MGF E肽能够促进ACL成纤维细胞胞外基质的合成与降解,进一步提高了细胞的迁移与侵袭,有助于组织损伤修复过程中成纤维细胞向损伤部位迁移,对ACL组织修复再生与术后康复具有积极的调控作用。

英文摘要：

Objective To explore the effects of mechano-growth factor（ MGF） E peptide on cell viability,migration and invasion of anterior cruciate ligament（ ACL） fibroblasts. Methods ACL fibroblasts were used in this study and（ 1） were treated with MGF E peptide（ 0,10 and 100 μg / L） for 24 h. Then,the medium was changed by 1% fetal bovine serum（ FBS）-low glucose DMEM medium. Cell activity,DNA content,cell apoptosis,matrix metalloproteinases-2（ MMP-2） and MMP-9 activity,type I collagen（ COL I） and type III collagen（ COL III）mRNA expression were measured after continued culture for 6 and 30 h;（ 2） were treated with MGF E peptide（ 0,5,10,20,50 and 100 μg/L） for 48 h. Then,cell activity and MMP-2 activity were verified. Cell migration and invasion of ACL fibroblasts were further tested by cell scratch test and transwell assay,respectively. Results（ 1） At 6 h,10 μg/L MGF E peptide significantly promoted MMP-2 and MMP-9 activities,but had no effect on cell viability,proliferation,apoptosis and mRNA expression of COL I and COL III. 100 μg / L MGF E peptide also significantly promoted MMP-2 and MMP-9 activities,as well as mRNA expression of COL I and COL III. However,it had no effect on the cell viability,proliferation and apoptosis. At 30 h,10 μg / L MGF E peptide significantly promoted MMP-9 activity and mRNA expression of COL I and COL II,but had no effect on cell viability,proliferation,MMP-2 activity and apoptosis. 100 μg / L MGF E peptide also significantly promoted MMP-9 activity and mRNA expression of COL III,but had no effect on the cell viability,proliferation,MMP-2 activity,cell apoptosis and mRNA expression of type I collagen.（ 2） MGF E peptide significantly promoted migration and invasion of ACL fibroblasts with dose-dependent manner in a certain degree,which might depend on the increase of MMP-2 activity. Conclusions MGF E peptide can actively accelerate synthesis and degradation of the extracellular matrix,further promote migration and invasion of ACL fibroblasts