中文摘要：

为揭示miRNA160 调控靶基因ARF 参与番茄叶片生长发育的作用机制,利用gateway 技术将miR160 前体转入pB7WG2D.1 表达载体,并通过农杆菌介导叶盘法获得35S：：SlmiR160 番茄转基因植株.同时使用荧光检测,分子及蛋白水平等鉴定方法筛选出转基因阳性株系.通过对T1代转基因植株的功能鉴定表明：超表达miR160 使番茄叶片宽度增加,叶面积增大,叶裂减少,暗示miR160 对番茄叶片发育有重要调控作用.

英文摘要：

For detailed study on the microRNA160 regulating target genes ARFs in tomato leaf growth developmental mechanism,using gateway technology which introduced miR160 precursor into pB7WG2D.1 expression vector, and through Agrobacteriummediated tomato leaf disc method to get the 35S：：SlmiR160 transgenic plants. At the same time, a variety of fluorescence detection,molecular and protein level identification method were used to filter out the genetic stability of transgenic strains. The functionalidentification of T1 transgenic plants showed that over expression of miR160 has an effect on the leaf width, size and less leaf lobes;compound leaves growth promisingly vegetative organs increased growth and development of tomato leaf. It suggests the regulation ofmiR160 on the molecular mechanism of tomato leaves development.