中文摘要：

目的本实验尝试制备阿霉素纳米脂质体并测定其包封率,探求其最佳制备工艺。方法采用薄膜分散高压均质法制备阿霉素纳米脂质体,高效液相色谱法检测阿霉素包封率。结果制得的阿霉素纳米脂质体大小均匀、分散性好、粒径在180nm左右、包封率为71.46%,4℃下保存3个月稳定。阿霉素在0.54μg/mL～21.60μg/mL范围内线性良好（r=0.9997）,回归方程为Y=46632x＋19140。结论该法制备的阿霉素纳米脂质体,工艺简单易行,质量可控。

英文摘要：

【Objective】This study attempted to prepare Doxorubicin nano-liposomes,to determine the entrapment efficiency,and explore the optimal process.【Methods】Doxorubicin nano-liposomes was prepared by thinfilm dispersion high-pressure homogenization.The HPLC was used to determine the entrapment efficiency of Doxorubicin nano-liposomes.【Results】The prepared Doxorubicin nano-liposomes were uniform in size,about 187 nm.The diameter dispersion was good.The encapsulation efficiency was 71.46%.The nano-liposomes could be stabilitily storaged for 3 months at 4℃.The Doxorubicin concentration had a good linearity （r =0.9997） in the range 0.54 μg/mL～21.60 μg/mL.The regression equation was Y=46632x＋19140.【Conclusion】The technique of preparing Doxorubicin nano-liposomes is simple and convenien and the method of quality control is simple and accurate.