中文摘要：

目的建立Folin-Cioca｜teu比色法测定尿液中总酚的方法。方法方法学建立。2013年12月至2014年4月选取北京医院123名健康志愿者，年龄18-81岁，男52名，女71名。尿液样本收集后加入终浓度0．5％HCl，分装，-80℃保存；尿样用PlexaPAX固相萃取柱进行提取纯化、洗脱液用氮气吹干后重组；Folin-Ciocaheu试剂显色，紫外分光光度计测定吸光度；没食子酸标准曲线计算尿总酚浓度，结果用尿肌酐进行校正。测定尿总酚浓度，分析总酚与血脂等心血管病危险因素的关系。相关性比较采用Spearman相关性分析。结果没食子酸标准溶液和尿液样本在0．5％HCl、避光条件下在室温可稳定48h、4℃稳定7d。PlexaPAX固相萃取柱对主要的多酚类物质有较好的回收率，并能有效去除维生素C、有机酸等物质的干扰。PAX抽提、Folin．Ciocalteu比色法测定尿液中总酚的平均批内变异和总变异分别为2．7％～3．8％和2．4％～4．6％。123名健康志愿者尿液中总酚浓度为114．13（82．97-146．70）mgGAE／gCrea。尿总酚与HDL-C（r=0．194，P=0．032）和apoAI（r=0．312，P〈0．001）呈正相关，与血尿酸（r=-0．220，P=0．014）呈负相关。结论成功建立了Folin-Ciocalteu比色法测定尿液中总酚的方法，方法精密、可靠，可用于评价多酚类物质的摄入水平，

英文摘要：

Objective To develop a solid phase extraction and Folin-Ciocaheu method for the measurement of total polyphenols in urine samples. Methods From a group of individuals attending an annual physical examination at Beijing hospital, 123 healthy volunteers （52 males and 71 females, ranging in age from 18 to 81 years） were recruited during the period from December 2013 to April 2014. Urine samples were stored in O. 5% I-IC1 at - 80℃. For analysis, samples were applied to the Plexa PAX solid phase extraction cartridge, to purity the polyphenols through washing, evaporating and reconstituting. Total polyphenols were measured by Folin-Ciocalteu colorimetric assay, calculated by gallic acid standard curve, and corrected by urine creatinine concentrations. The relationship between total polyphenols and fruits and vegetable intake and cardiovascular disease risk factors were analyzed. Results Gallic acid standard solution and urine samples were stable in 0. 5% HC1 for 48 h at RT and 7days at 4℃, respectively. The PAX cartridge effectively eliminated the possible interfere materials in urine and had better recovery for most of the polyphenol types. The inter assay and total CVs for the measurement of total polyphenols were 2. 7% - 3.8% and 2.4% - 4. 6 %, respectively. Total polyphenol concentrations of 123 healthy subjects were 114. 13（82. 97 -146. 70）rag GAE/g Crea. Total polyphenol levels positively correlated with both HDL-C （r =0. 194, P =0. 032） and apoAI （r = 0. 312,P 〈0. 001 ）, and negatively correlated with serum uric acid levels （r = -0. 220,P = 0. 014 ）. Conclusions We established a measurement of total polyphenols in urine samples using solid phase extraction and Folin-Ciocalteu method. This simple, precise method reliable and may be use to assess dietary polyphenols intake. （Chin J Lab Med,2015,38：178-182）