中文摘要：

为了研究狂犬病毒作为载体表达外源基因的特性，将禽流感病毒HA基因插入狂犬病毒全基因组cDNA感染性克隆pHEP-3．0G基因与L基因的假基因位点，再通过反向遗传技术，获得了携带流感病毒HA基因的嵌合狂犬病毒（HepFHA），并进行了病毒传代与HA基因表达的研究．结果显示，重组病毒经BHK-21细胞传代10次，病毒表达稳定，但各代次常规红细胞凝集试验均为阴性；Western—blotting检测到病毒表达产物中存在部分HA蛋白的表达，其相对分子质量为39540，与HA裂解的HA1蛋白相对分子质量相吻合．

英文摘要：

To study the characteristics of rabies virus as vector, hemagglutinin gene of avian influenza virus was inserted at the site of pseudogene between G and L genes and a chimeric virus, designated as HepFHA, was generated by the technique of reverse genetics. The virus HepFHA grew well in BHK-21 cells after passage for 10 times, but did not show hemagglutination. The foreign protein was analyzed by SDS-PAGE and Western-blotting, and the results indicated that the partial protein of hemagglutinin was expressed with relative molecular mass of 39 540, identical to that of HA1.