中文摘要：

目的对椰子花粉的变应原组分进行初步的分离、鉴定及纯化。方法提取椰子花粉粗提液,用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分离椰子花粉的蛋白质组分并测定其相对分子质量,采用免疫印迹法鉴定其变应原成分,并通过离子交换层析对椰子花粉变应原进行初步分离纯化,免疫印迹进行检测。结果SDS-PAGE显示椰子花粉粗提液有10条蛋白带,其中相对分子质量(Mr)为60 000、50 000、35 000、28 000、19 000、16 000和14 000的蛋白可与椰子花粉过敏性病人血清IgE结合,且Mr50 000、16 000和14 000为主要变应原;离子交换层析结果显示主要过敏原成分主要分布在Ⅴ峰中。结论对椰子花粉变应原进行了初步的分离、鉴定和纯化,为临床椰子花粉变态反应疾病的诊断和治疗奠定了基础。

英文摘要：

Objective To purify and characterize allergenic components of coco pollen.Methods Coco pollen protein was extracted and analyzed by SDS-PAGE.Coco pollen extracts were analyzed by immunoblotting for characterizing the allergenic components.The allergenic components were separated by ion-exchange chromatography with DE-52,and then the products of the ion-exchange chromatography were characterized by Western-blotting.Results SDS-PAGE analyses suggested that the extract of coco pollen displayed 10 protein bands...