中文摘要：

为了了解松辽黑猪肌内脂肪沉积的分子遗传学机理,利用基因芯片技术对松辽黑猪群体内背最长肌肌内脂肪含量高和低的个体进行了转录分析,利用实时定量PCR对部分转录差异基因进行了检测。差异表达分析显示,共有153个基因显著变化;差异基因经GO分类显示,主要参与细胞生长与死亡、信号分子及互作、信号传导、氨基酸代谢、脂类代谢等生物学过程;所验证的IGF2、PRKAG3、UCP3基因与芯片结果基本一致。本研究为深入了解松辽黑猪肌内脂肪沉积的机理奠定了基础。

英文摘要：

In order to investigate the molecular mechanisms underlying porcine intramuscular fat deposition, the transcriptional profile of longissimus dorsi muscle between lower intramuscular fat content individuals and higher individuals were compared by microarrays in Songliao Black Swine, and the differentially expressed genes were verified by real-time PCR. The results showed that 153 differentially expressed genes were found between the two groups. Differentially expressed genes classified by GO （Gene Ontology） analysis mainly involved in cell growth and death, signaling molecules and interaction, signal transduction, metabolism of amino acids, lipid metabolism etc. In addition, the Real-time-PCR results of IGF2, PRKAG3, UCP3 were consistent with that of gene chip. These results would offer the basic data for the further study on the molecule genetic mechanism of intramuscular fat deposition in Songliao Black Swine.