中文摘要：

制备乳糖酰化壳聚糖基自组装纳米微粒,用作抗肿瘤药物—甲氨喋呤（MTX）的载体,为肝靶向药物载体系统的研究提供体外参考数据。合成N-乳糖酰化-N-胆甾醇琥珀酰基壳聚糖（CLCS）,并通过红外（IR）及核磁共振氢谱（1 H NMR）对其进行结构确证。采用自组装的方法制备CLCS纳米微粒,并通过透射电镜及动态激光散射法对纳米微粒的形态结构和粒径大小进行表征。采用超微透析法实现CLCS纳米微粒对MTX的包载,紫外分光光度法检测MTX的包封率和载药量,并通过动态透析实验考察载药CLCS纳米微粒的体外释药特征。

英文摘要：

This study is to prepare lactosylated chitosan-based nanoparticles used as the carrier for the anticancer drug：methotrexate（MTX） and to provide the reference data for the study of liver-targeting drug carrier systems.Firstly,N-lactosyl-chitosan conjugate（CLCS） was synthesized and characterized by FT-IR and 1H NMR.Secondly,CLCS nanoparticles were prepared by the self-assemble method,and their size and morphological characteristics were analyzed by dynamic laser light scattering and transmission electron microscopy.Thirdly,MTX was loaded into CLCS self-assembled nanoparticles using microdialysis method;MTX loading efficiency and loading content were determined by UV method;MTX release properties from CLCS self-assembled nanoparticles were valued by the dynamic dialysis method.