中文摘要：

目的探讨激活素A（Activin A,ActA）对细胞滋养层细胞凋亡的调节作用. 方法用ActA刺激无血清培养的7～8周人绒毛细胞滋养层细胞后,激光共聚焦和蛋白印迹分析检测凋亡信号通路相关蛋白的表达变化,TUNEL检测细胞凋亡情况.结果高浓度ActA（50 ng/ml）作用24 h后,人绒毛细胞滋养层细胞中TUNEL阳性信号显著增强;同时Caspase-3,-9表达量明显提高（对照组：0.13±0.048和0.26±0.004;50 ng/ml组：0.34±0.068和1.54±0.062;100 ng/ml组：0.45±0.091和0.58±0.008）,Caspase-8表达亦略有增加;凋亡相关蛋白P53（对照组：5.2±1.02;50ng/ml组：12.3±1.91;100 ng/ml组：11.5±1.73）和Bax（对照组：0.09±0.021;50 ng/ml组：0.46±0.065;100 ng/ml组：0.68±0.142）表达水平显著增加.结论高浓度ActA可诱导人细胞滋养层细胞发生凋亡,其作用可能主要是通过细胞凋亡的线粒体途径来实现的.

英文摘要：

Objective To study the effect of activin A （ActA） on the regulation of cell apoptosis in human cytotrophoblasts in the early trimester. Methods Human cytotrophoblasts derived from placental villi at 7-8 gestational weeks were cultured in serum-free system, and treated with ActA. Cell apoptosis was detected by TUNEL, and the expression of apoptosis signaling molecules （including Caspase-3, -8 and-9） and apoptosis-related proteins （P53 and Bax ） were detected by Confocol microscopy and Western Blot analysis. Results Treatment of ActA at high concentration （50 ng/ ml） for 24 h significantly induced cell apoptosis in human cytotrophoblasts. The expressions of Caspase-3 and Caspase-9 were obviously up-regulated（control group ： 0.13 ± 0. 048 and 0.26 ± 0. 004 ; 50 ng/ml group：0.34±0. 068 and 1. 54±0.062;100 ng/ml group：0.45±0.091 and 0. 58±0.008）, while the level of Caspase-8 increased slightly, Meanwhile, the expressions of P53 （control group：5. 2 ±1.02;50 ng/ml group：12.3±1.91;100 ng/ml group：11.5±1.73）and Bax （control group：0.09± 0.021;50 ng/ml group：0. 46±0.065;100 ng/ml group：0.68±0.142） protein in the cytotrophoblasts were strongly stimulated by ActA, Conclusions High concentration of ActA can induce cell apoptosis in human cytotrophoblasts which might be mediated by the mitochondria signaling pathway.