采用对峙培养法和含毒介质法测定67-1菌株和无菌发酵液对水稻纹枯病菌的抑制作用和对菌核形成的影响;测定67-1菌株孢子悬浮液和无菌发酵液处理不同时间对菌核萌发的抑制作用;采用种子处理和土壤处理的方法测定67-1菌株孢子悬浮液对水稻幼苗生长的影响;通过室内盆栽试验和田间药效试验,测定67-1菌株孢子粉对水稻纹枯病的防病效果。结果表明,67-1菌株及其无菌发酵液对水稻纹枯病菌的菌丝生长、菌核的形成有明显的抑制作用,发酵液60℃处理30min后抑菌率有所下降,121℃处理30min抑菌活性丧失;孢子悬浮液和无菌发酵液处理不同时间菌核萌发率明显下降;67-1菌株孢子悬浮液土壤和种子处理后,能够提高水稻发芽率,促进水稻的生长;750g/hm2的孢子粉对水稻纹枯病有较好的防治作用,田间试验防病效果和保产效果分别达69.1%和22.4%,与20%井冈霉素可湿性粉剂没有显著差异。
We detected pretection and theraphy effects of Gliocladium roseum 67-1 to Rhizoctonia solanii.The results indicated that 67-1 strain and filtered fermentation had obvious inhibition effects on hypha's elongation and sclerotium's form of R.solanii strain,and inhibition efficiencies decreased after bathed for 30 minutes under 60℃,while inhibition effects decreased to zero after bathed for 30 minutes under 121℃.Sclerotium germination rate decreased after treated with spore suspension and filtered fermentation.Spore suspension increased rice germination percentage and promoted rice growth.The field tests indicated 750g/hm2 spore powder had better control effects on rice sheath blight,and prevention efficiency and guaranteed efficiency were 69.1% and 22.4%,respectively,which had no obvious difference with 20% validamycin.