中文摘要：

通过RT-PCR和RACE的方法得到了编码‘津田’芜菁（Brassica rapa L. ssp. rapifera‘Tsuda’）BrCOP1的cDNA序列，长2 034 bp，编码677个氨基酸，与拟南芥同源性达94%，命名为BrCOP1，GeneBank收录号为JF738111。Northern杂交分析表明BrCOP1的表达没有组织特异性，而在整株幼苗及成熟植株肉质根表皮中受长波紫外线（UV-A）诱导表达，但成熟植株的直根表皮中表达量不随处理时间延长而增加。这暗示BrCOP1作为光控发育的重要调控因子，可能在UV-A诱导‘津田’芜菁花青素合成及光信号转导中起重要作用。

英文摘要：

The COP1 gene（BrCOP1，accession number JF738111）was isolated from total RNA of Brassica rapa L. ssp. rapifera‘Tsuda’by RT-PCR and RACE. BrCOP1 cDNA contained an opening reading frame of 2 034 bp，encoding a predicted protein of 677 amino acid residues. The deduced amino acid sequence of BrCOP1 shared 94% identity with Arabidopsis thaliana COP1. Northern blotting results demonstrated that expression of BrCOP1 exhibited no tissue specificity，and had no obvious difference in seedlings under various treatments but was induced by UV-A light，while the expression of BrCOP1 was induced after exposed to UV-A for 6 hours in the swollen hypocotyls，however expression level was not increased with the prolongation of time. The above results suggested that BrCOP1，as a key factor of light-dependent development，might play a crucial role in UV-A induced anthocyanin biosynthesis and light signal transduction.