中文摘要：

考察了NKA-9、S-8、XDA-1、AB-8、HPD-100、HPD-600 6种大孔吸附树脂对宁夏枸杞总黄酮的吸附和解吸性能,筛选出XDA-1树脂的效果最佳;考察了pH值、样液浓度、流速等对XDA-1树脂静态吸附效果的影响;并进行了动态吸附试验,确定出XDA-1树脂动态吸附枸杞总黄酮的最佳条件：样品液浓度为0.25mg/mL,控制流速为0.5mL/min,样品液pH 5;最佳洗脱条件：洗脱液为80%的乙醇水溶液,用量为35mL（5倍柱床体积）。在此条件下,枸杞总黄酮含量从27.2%提高到79.8%,回收率为87.4%,表明XDA-1树脂可用于宁夏枸杞总黄酮的分离纯化。

英文摘要：

Six macroporous resins,including of NKA-9、S-8、XDA-1、AB-8、HPD-100 and HPD-600,were studied on separation and purification of total flavonoids in Lycium Barbarum Linnaeus.The experimental results showed XDA-1 resin had better adsorption and desorption performance than other resins.With XDA-1 resin,the effects of pH、extract concentration and flow rate on the adsorption performance were studied.The dynamic adsorption and desorption conditions of XDA-1 resin for total flavonoids were optimized.The optimum dynamic adsorption conditions for total flavonoids were obtained as follows： total flavonoids concentration,0.25mg/mL;pH,5;flow rate,0.5mL/min.The optimum desorption conditions were obtained at eluant,80% ethanol aqueous solution（v/v）;the amount of elution,35mL（5 bed volume）.In these techniques,total flavonoids content were increased from 27.2% to 79.8%,with a total flavonoids recovery rate of 87.4%.The results indicated that XDA-1 resin could be used in separation and purification for total flavonoids in Lycium Barbarum Linnaeus.