中文摘要：

构建离子液体1-乙基-3-甲基咪唑醋酸盐[EMIM]OAc与纤维素的均相体系,采用酶原位降解法,以3,5-二硝基水杨酸法表征降解效果,考察各因素的影响确定最佳工艺条件：6.6 g 3%（质量分数）原溶液与4 mL pH4.6柠檬酸钠缓冲溶液组成均相体系、6 mg酶、50℃、26 h,酶解率达78.5%,比非均相下提高了近2倍。红外光谱（FT-IR）表征结果表明,离子液体在溶解纤维素的过程中破坏纤维素中的氢键,纤维素的结晶形态由结晶态变为无定型态,增加酶与纤维素的接触位点,提高反应的酶解率。

英文摘要：

The enzymatic degradation of microcrystalline cellulose in the homogeneous system of 1-ethyl-3-methylimidazolium acetate（OAc） and cellulose was investigated.The influence of reaction conditions on the results of degradation was examined by using 3,5-dinitrosalicylic acid（DNS） characterization method to detect the result of degradation.The optimum condition for degradation is obtained as follows： 6.6 g 3% cellulose in ionic liquid solution,4 mL pH4.6 citric acid-sodium citrate buffer solution,enzyme 6 mg,reaction temperature 50 ℃ and reaction time 26 h.Under the above condition,the enzymatic rate is 78.5%.It is also found that ionic liquid OAc has good cellulose solubility and biocompatibility,which is efficient to promote the enzymatic degradation of cellulose.