中文摘要：

对选育的一株木腐真菌Psathyrella candolleana进行液体深层发酵生产漆酶，通过硫酸铵盐析和离子交换色谱等纯化技术分离发酵酶液后，得到纯度较高的漆酶，并对其酶学性质进行研究．结果表明：P．candolleana在发酵罐中的漆酶产量最高可达9100U／L；分离纯化后得到漆酶活性组分，其比活力达到438．0U／mg，纯化倍数为13．2，得率为50．1％；该漆酶蛋白分子质量约为61．2ku，米氏常数为16μmol／L；该漆酶催化反应最适温度和pH值分别为45℃和5．0，在4～10℃和pH9．0的条件下漆酶活力稳定性较好，Mg2＋、Mn2＋、Zn2＋等金属离子对其活力的影响较小．

英文摘要：

Laccase from a newly isolated wood-rot fungus Psathyrella Candolleana, was produced via the submerged fermentation, and high-purity laccase was obtained by means of the ammonium sulfate precipitation and the ion-exchange chromatography. Then, the enzymatic properties of the laccase were also analyzed. The results indicate that the maximal activity of the laccase from P. candolleana in the fermentor reaches 9100 U/L, that the active component of the lacease achieved after 13.2-fold purification is of a specific activity of 438.0 U/rag with a yield of 50. 1%, that the molecular mass and Michaelis constant of the laccase are respectively about 61.2ku and 16 μmol/L, and that the optimal pH value and temperature for the laccase activity are 5. 0 and 45 ℃, respectively. Moreover, it is also found that the laccase activity is stable under 4 - 10℃ at pH 9.0 and is not sensitive to Mg2＋, Mn2＋ and Zn2 ＋.