中文摘要：

目的：克隆人IL-37b基因eDNA，并构建其真核表达载体。方法：从包皮组织中提取总RNA，用逆转录-PCR技术，扩增人IL-37b基因全长编码区片段，经酶切后，克隆入pcDNA3．1（＋）载体中，构建真核表达载体pcDNA3．1-hIL-37b，用内切酶双酶切鉴定与DNA测序。结果：获得的IL-37b基因序列与GenBank数据库中的一致。人IL-37b基因的全长编码片段为657bp，翻译为218个氨基酸。结论：人IL-37b基因成功地克隆并构建了其真核表达载体，为进一步进行IL-37的表达与功能研究奠定了基础。

英文摘要：

Objective：To clone the gene sequence encoding human interleukin （IL） -37b gene and construct its eukaryotic expression vec- tor. Method：Total RNA was extracted from the human prepuce and the gene fragment encoding IL -37b gene was amplified by reverse transcriptase - PCR using specific primers and then cloned into pcDNA3 vector. The eukaryotic expression plasmid pcDNA3.1 - hIL -37bwas constructed and transformed into the E. coli for identification. The inserted IL -37b cDNA was sequenced. Result： DNA sequencing showed that the eDNA of IL - 37b had the full length of human IL - 37b gene consists of 657 bp encoded the product of 218 amino acids. The eDNA shared a high nucleotide homology with that reported previously. Conclusion： We have successfully cloned the full - length eDNA of human IL -37b, which has paved the way for further studying on its biological function.