中文摘要：

建立优化的转化条件，将Mu转座复合物电转化到临床分离的-株铜绿假单胞菌（Pseudomonas aeruginosa）PA68中，最高转化效率达3．66×10^4 CFU／μg DNA．通过表型筛选，得到三株鞭毛运动能力缺陷的突变子，Sournthern杂交证实转座子为单点插入．经基因克隆、核苷酸测序研究，证明转座子分别插入到uvrD、phzF1、zwf，三个基因中，这是首次在国际上将Mu转座重组技术应用到鞭毛运动相关基因的研究中．由于人工Mu转座技术具有随机单点插入的优点，克服了传统转座子能在染色体上迁移的缺点，为进一步研究P．aeruginosa的鞭毛运动机理及致病性奠定基础．

英文摘要：

In this study, Mu transposition recombination technique was used to study a cluster of genes required for swimming motility mediated by flagellum of P. aeruginosa. Optimum conditions including growth state of the strain, electroshock voltage were studied for the electroporation of a clinical isolate strain PA68 with Mu transposonase complexes, and get highest transformation efficiency. Three swimming motility deficient mutants were isolated. Mini-Mu transposon inserted into genomic DNA in a single copy, which was confirmed by Southern blotting. Gene cloning and sequencing of the region flanking the insertion revealed that mini-Mu integrated into gene uvrD, zwf and phzF1 respectively. Mu transposon recombination technique is a new efficient method to study the function of bacterial genome, it＇s first time to be utilized to study the genes involved in swimming motility of P. aeruginosa.