中文摘要：

为了阐明临床治疗异常黑胆质性疾病有效的成熟剂和清除剂的作用机理，以H2O2诱导淋巴细胞的氧化应激模型，在细胞和分子水平上研究2种复方对氧化应激的相关蛋白表达、细胞凋亡的影响。采用western-blot研究淋巴细胞Bcl-2、NF-KB的表达；用荧光显微镜的双苯咪唑（Bisbenziimide）类Hoechst33258法检测分析凋亡指数、评价细胞凋亡的情况。结果显示，异常黑胆质成熟剂能够上调Bcl-2蛋白的表达。而清除剂能够下调该蛋白的表达，二者的作用相反。异常黑胆质成熟剂和清除剂能显著地抑制H2O2诱导的NF-KB激活，而前者本身却能促进NF-KB的活化，并且能抑制氧化应激诱导的淋巴细胞凋亡。由此得出结论：H2O2诱导淋巴细胞氧化应激模型的体外实验系统是研究传统医药复方生物效应信息的途径之一；异常黑胆质成熟剂及清除剂对氧化应激的淋巴细胞Bcl-2蛋白表达、NF-KB激活以及细胞凋亡的作用明显不同。提示两种复方临床应用有效性的作用机制与氧化应激诱导的细胞凋亡有关。

英文摘要：

This paper studies the effect of Abnormal Savda Munziq and Mushil on H202-induced lymphocyte Bcl-2 protein expression, NF-KB activation and apoptosis, western-blot was employed to determine cytosol and nucleous NF-KB p65 protein. Hoechst 33258 fluorescence dying technique and fluorescence microscopy were used to evaluate apoptosis after treatment of Abnormal Savda Munziq and Mushil on H202 induced lymphocyte cultures. Bcl-2 protein was found significantly up-regulated by Abnormal Savda Munziq application while Abnormal Savda Mushil treatment shows an opposite effect. Both Abnormal Savda Munziq and Mushil can inhibit H202-induced NF-KB activation and Mushil itself displays activation effect of lymphocyte NF-KB. Abnormal Savda Munziq can also inhibit HzOz-induced lymphocyte apoptosis. The H2O2-induced oxidative stress lymphocyte cell culture is a good experimental model to investigate the multibiological effects of traditional medicine; different effects of Abnormal Savda Munziq and Mushil on the Bcl-2 protein expression, NF-KB activation and lymphocyte apoptosis suggest that the mechanism of Abnormal Savda Munziq and Mushil＇s effectiveness in clinical application may be associated with apoptosis process.