目的采用酸性氧化电位水(EOW)和碱性还原电位水(ERW)研究对枯草杆菌黑色变种芽胞(ATCC9372)和大肠杆菌(8099)的杀菌效率和杀菌机制。方法利用离子膜电解法制备EOW和ERW。采用悬液定量杀菌实验。结果当杀菌时间为30min时,EOW对枯草杆菌黑色变种芽胞杀菌率为99.59%,杀灭对数值为2.38log cfu/ml;当杀菌时间为60min时,ERW对枯草杆菌黑色变种芽胞杀菌率为94.62%,杀灭对数值为1.27log cfu/ml;当杀菌时间为30min时,ERW对大肠杆菌杀菌率达到100%,杀灭对数值为8.26log cfu/ml。当EOW中有效氯(ACC)值为74.90mg/L时,对枯草杆菌黑色变种芽胞杀菌率为99.89%,杀灭对数值2.67log cfu/ml;而当ACC值降为6.82mg/L时,其杀菌率仅为83.30%,杀灭对数值仅为0.78log cfu/ml。另外,当高氧化还原电位值(ORP)值为1138mV,pH值为2.24时,EOW对枯草杆菌黑色变种芽胞杀菌率为99.99%;当ORP值变化为883mV,pH值为5.43时,其杀菌率降为99.73%;两者差距不大,但两者杀灭对数值相差1.30log cfu/ml。此外,当ORP值为-918mV时,ERW对枯草杆菌黑色变种芽胞杀菌率为94.62%,杀灭对数值为1.27log cfu/ml;而当ORP值为-155mV时,其杀菌率降为40.19%,杀灭对数值降为0.13log cfu/ml。结论通过考察,我们得出EOW杀菌机制是以化学因素(ACC)杀菌为主,物理杀菌为辅。ERW杀菌是物理杀菌,ORP值对杀菌效果影响很大。
Objective To study the sterilizing effect and mechanism of electrolyzed-oxidizing water (EOW) and electrolyzed-reductive water (ERW) for Bacillus subtilis var. niger (ATCC9372) and Escherichia coli (8099). Methods The generations of EOW and ERW were made in the ion membrane electrolysis cell. The sterilization manner was the suspension quantitative germicidal test. Results The killing rate of EOW for Bacillus subtilis var. niger was 99. 59% in 30 minutes and the killing logarithm value was 2. 38 log cfu/ml ; the killing rate of ERW for Bacillus subtilis var. niger was 94. 62% in 60 minutes and the killing logarithm value was 1.27 log cfu/ml ; the killing rate of ERW for Escherichia coli was 100% in 30 minutes and the killing logarithm value was 8.26 log cfu/ml. When the available chlorine content (ACC) value in EOW was 74. 90 mg/L and killing time was 30 minutes, the killing rate for Bacillus subtilis var. niger was 99. 89% and the killing logarithm value was 2. 67 log cfu/ml. When the ACC value was 6. 82 mg/L, the killing rate for Bacillus subtilis var. niger was 83.30% and the killing logarithm value was 0. 78 log cfu/ml under the same time. When the oxidizing-reductive potential(ORP) and pH values of EOW were 1138 mV and 2. 24 respectively, the killing rate for Bacillus subtilis var. niger was 99. 99%. When the ORP and pH values of EOW were 883 mV and 5.43 respectively, the killing rate of Bacillus subtilis var. niger was 99. 73%. When the ORP value of ERW is -918 mV, the sterilizing rate for Bacillus subtilis var. niger was 94. 62% ; when the ORP value is - 155 mV, the sterilizing ratio was only 40. 19%. Conclusion It indicates that the sterilizing mechanism of EOW is mainly chemical processes (ACC), while the physical factors are auxiliary. The sterilizing mechanism of ERW is physics sterilizing that the mainly factor is ORP.