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中文摘要：
甜菜碱在植物体内以胆碱为底物，经两步酶催化反应合成，其中催化第二步反应的是甜菜碱醛脱氢酶（Betaiae Aldehyde Dehydrogenase，BADH）．为研究甜菜碱醛脱氢酶基因在酵母中是否具有生物学功能，通过PCR-RACE技术从红树植物白骨壤（Avicennia marina）中分离得到BADH基因，将其转入酿酒酵母AH109中．通过对重组酵母生长曲线的测定，发现重组酵母对NaCl的耐受度由转化前的9％提高到14％，表明白骨壤BADH基因在酵母中能得到有效表达出具生物学活性的蛋白质．
英文摘要：
The synthesis of betaine in plants is two-step oxidation reaction of choline, and the the second step is catalyzed by betaine aldehyde dehyclrogenase （BADH） . In order to study the function of BADH gene in yeast,the BADH in Avicennia marina was obtained with PCR technique and transformed into Saccharomyces cerevisiae AH109. The salt tolerance of recombinant yeast increased from 9% to 14% by reorganizing the recombinant yeast＇s growth curve. The results showed that the BADH gene can be effectively produce protein with biological activity in the recombinant yeast.
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