中文摘要：

针对蛋白质离子交换层析分离过程预测的困难,选择zeta电位作为中间参数,建立zeta电位与层析容量因子之间的关联。选择牛血清白蛋白（BSA）作为模型蛋白质,考察了pH、离子浓度、离子种类等对蛋白质zeta电位的影响,证实了zeta电位作为蛋白质表面电荷特性表征参数的可行性。以阴离子交换Sepharose FF DEAE为模型层析介质,测定了BSA在不同pH和盐浓度下的容量因子。综合考虑蛋白质和介质两方面因素,选择两者zeta电位乘积（-ζ1×ζ2）作为中间参数,对于不同pH和盐浓度的流动相,（-ζ1×ζ2）与容量因子的对数呈现良好的线性关系,实现了由简便的zeta电位分析预估宏观离子交换层析分离行为的目的,该关联关系的建立将有助于层析分离过程的优化设计。

英文摘要：

Aiming at the difficulty of predicting separation behavior of proteins in ion exchange chromatography, the zeta potential was introduced as an intermediate parameter to correlate the retention factor in ion exchange chromatography. Bovine serum albumin （BSA） was used as model protein, and the influences of pH, ionic concentration and ionic types on the zeta potential of BSA were investigated. The results indicate that zeta potential is useful and feasible parameter to characterize the surface charge properties of protein. With typical anion exchanger of Sepharose FF DEAE as model adsorbent, the retention factors of BSA under varying pH and ionic concentration were measured by isocratic elution method. Considering the impacts of both protein and adsorbent, the product of zeta potential of protein and zeta potential of adsorbent was chosen as the intermediate parameter, （-ζ1×ζ2）. It was found that there is a good linear relation between the parameter （-ζ1×ζ2） and the logarithm of retention factor under different pH and ionic concentrations. The relationship obtained in the present work could be used to predict the separation behavior of protein in ion exchange chromatography with zeta potential measurement, which would certainly improve the optimizing design of chromatography process.