为筛选分解高效氯氰菊酯的优良菌株,试验从活性污泥中分离出5株菌株,均能一定程度地降解高效氯氰菊酯。通过液相色谱分析,菌株XCH-5降解高效氯氰菊酯的能力最强。观察该菌株的形态学特点,再结合测序分析,可鉴定XCH-5菌株为栖土曲霉。利用正交法优化降解条件,得出该菌株在最优水平组合为接种量0.2 g/L、温度35℃、p H7.5的情况下,10 d对200 mg/L高效氯氰菊酯的降解率可达到78%。
In order to screen new microbial strains with high degrading capabilities for beta-cypermethrin from agricultural by-products, five strains were isolated from the activated sludge. By liquid chromatography analysis, a strain named XCH-5 exhibited the strongest degradation ability among these five strains. Further investigation revealed that XCH-5 was Aspergillus terricola as showed by morphological characteristics and ITS sequencing analy- sis. By orthogonal design method, the optimal combinations of the strain XCH-5 for beta-cypermethrin degradation were found with inoculation amount of 0.2 g/L, temperature 35℃, and pH 7.5. Under the optimized conditions, XCH-5 showed 78% of degradation rate for beta-cypermethrin after ten days of treatment.