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豨莶草提取物抗多柔比星致大鼠急性心肌损伤的作用

ISSN号：1004-0781
期刊名称：《医药导报》
时间：0
分类：R285[医药卫生—中药学;医药卫生—中医学]
作者机构：[1]河北医科大学第一医院药剂科,石家庄050031, [2]河北医科大学基础医学院药理教研室,石家庄050017, [3]河北省计划生育科学技术研究院生殖医学中心,石家庄050050
相关基金：国家自然科学基金资助项目（81273600）; 河北省中医药管理局基金资助项目（2012008）

作者：于静[1,2], 王婧[1], 苏素文[2], 谢克让[2], 张玉[3]

关键词：豨莶草, 多柔比星, 心肌损伤, 氧化应激, Herba siegesbeckiae , Doxorubicin , Cardiac injury , Oxidative stress

中文摘要：

目的探讨豨莶草（HS）提取物对多柔比星（DOX）所致大鼠急性心肌损伤的影响。方法将40只大鼠随机分为4组,每组各10只：高、低剂量药物组分别灌胃给予HS提取物170和340 mg.kg-1,对照组、模型组灌胃给予等容积纯化水,连续7 d。给药第5天,除对照组腹腔注射0.9%氯化钠溶液外,其他3组均腹腔注射DOX 20 mg.kg-1。于第7天将大鼠麻醉后记录心电图（ECG）的变化,测定血清各种生化指标、过氧化产物及自由基的含量,心肌组织过氧化产物及自由基的含量。结果与模型组比较,高、低剂量药物组对DOX所致S波、T波幅度加深有明显的恢复作用,高剂量药物组尚可使心率部分恢复,但对QT间期未见明显恢复作用。高、低剂量药物组血清中肌酸磷酸激酶（CK）、肌酸激酶同工酶（CK-MB）、总胆固醇（TC）、乳酸脱氢酶（LDH）水平均较模型组低,血清及心肌组织中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）及丙二醛（MDA）水平均较模型组有所恢复。心肌组织病理结果显示高、低剂量药物组不同程度缓解DOX所致的炎细胞浸润,以高剂量组作用最为明显。结论 HS明显减轻DOX所致大鼠的急性心肌损伤,其作用机制可能与HS提取物能增强机体抗氧化应激能力有关。

英文摘要：

Objective To investigate the potential protective effect of Herba siegesbeckiae （HS） extracts on cardiac injury induced by doxorubicin （DOX） in rats. Methods A total of 40 male Sprague-Dawley rats were randomly divided into 4 groups with 10 rats in each. The low and high dose group were given with HS by intragastric administration at the doses of 170 and 340 mg · kg^-1 , respectively, and the control and model group were given with the equal amount of water for 7 consecutive days. On the 5th day, all groups were given DOX（ 20 mg· kg^-1） intraperitoneally except the control group, which was given equal amount of saline . The peroxidative lesions were evaluated by both biochemical and histopathological methods 48 h after DOX administration. On day 7, evaluation of cardioprotective effect of HS was performed by analyzing the electrocardiographic （ECG） parameters, serum biochemical indicators and contents of preoxidation （MDA） and free radicals in both serum and cardiac tissue. Results HS reversed the abnormal changes of ECG caused by DOX, except for the prolongation of QT interval. Pretreatment with HS showed significant reduction of serum TC, LDH, CK and CK-MB levels in a dose-dependent manner compared with the DOX group. The increase of MDA production and decrements of SOD and CAT caused by DOX were also recovered dose dependently in HS treated groups. Histopathological inspection of the left ventricle showed reduction of inflammation cells in peripheral vessels in the HS groups compared with the D0X group. Conclusion HS mitigates acute cardiac injury induced by D0X in rats,which may relate to its anti-oxidative effect.

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