采用微弱发光检测系统,研究了血清的微弱发光。测试了健康人、高血糖和高血脂病人血清样品的微弱发光,探讨了血清的发光强度随血清中血糖和三酰甘油浓度变化的规律,并用编制的软件,实现了血清微弱发光图像的定量分析。实验结果表明,血糖浓度增加会导致血清的发光强度单调递增,微弱发光图像的灰度值增加;三酰甘油血清发光图像的灰度值也随着血脂浓度的增加而变大。与血糖相比,血清发光强度随三酰甘油浓度变化的效果不是十分明显。因此,可以采用血清的发光强弱来判断血清中血糖含量是否超标,此方法可有效地分析血清中血糖是否异常。
The low luminescence of serum is tested with the low luminescences detection system. In the experiment the low luminescence of serum of the hyperglycemia, hyperlipoidemia, and normal man are tested respectively, and the laws of serum radiation intensity with variation of blood sugar and triglyceride concentration are studied. And the image of the low luminescence of serum is analyzed quantitatively with self-programmed software. The experimental result shows that the serum radiation intensity increase synchronously with blood sugar concentration linearly. The gray threshold of triglyceride luminescence image also increases with triglyceride concentration, but it is not obviously as the former one. So the blood sugar concentration can be estimated by the serum luminescence intensity, and it is effective. It provides experimental foundation for the medical diagnose.