子囊盘在地衣尤其是壳状地衣的分类中具有重要意义,其特征可通过光学显微镜进行观察。但是,一些地衣如茶渍属(Lecanora)、黄茶渍属(Candelariella)等属的子囊盘中的油滴常影响子囊孢子中细胞数目的观察。本实验用KOH溶液和苏丹Ⅲ溶液对子囊盘切片进行处理,结果表明KOH溶液可以去除油滴,而苏丹Ⅲ溶液能对油滴着色,使其呈橘黄色,并且这两种溶液都不会破坏细胞的结构。因此这两种溶液都能有效排除观察过程中油滴的干扰。
Apothecium, which can be described using light microscope, is very important in the taxon of lichens, especially in crustose ones. But the apothecium some lichen genera, such as species of Lecanora and Candelariella, contains oil drops that make you count how many cells per spore has by error. In this paper, the authors designed the experiment to deal with the apothecial sections by KOH solu- tion and Sudan Ⅲ solution. The result shows that KOH solution can wipe off oil drops, and Sudan Ⅲ solution can stain them orange-yellow, but both of the solutions have no effect on the spores. Therefore, the two methods can eliminate interference of oil drops during the observation effectively.