中文摘要：

利用制备型HPLC对泰和乌骨鸡活性肽进行分离,流动相为0.01mol/L的磷酸盐溶液,采用强酸型离子交换树脂和强碱型离子交换树脂两步脱盐法对泰和乌骨鸡活性肽各分离组分进行脱盐处理。以肌肽和抗坏血酸为对照,通过清除羟自由基作用、抑制脂质过氧化作用以及对Fe2＋和Cu2＋螯合能力4个体系测定泰和乌骨鸡活性肽及其分离组分的体外抗氧化能力。结果表明：泰和乌骨鸡活性肽分离所得13个组分均具有一定的抗氧化能力,且与质量浓度呈量效关系,绝大部分分离活性肽组分比活性总肽有更强的羟自由基清除能力、脂质过氧化抑制能力以及Fe2＋螯合能力。

英文摘要：

The Taihe black-bone silky fowl muscle hydrolysate prepared by papain hydrolysis were separated to obtain active peptides by preparative HPLC with 0.01 mol/L phosphate buffer as the mobile phase,and the separated fractions were then pooled and desalted stepwise by 001 × 16 strong acid cation exchange resin and 201 × 7 strong basic anion exchange resin column chromatography. The hydrolysate and its separated fractions were tested for their in vitro antioxidant effects by hydroxyl free radical scavenging array,lipid peroxidation inhibition assay and measuring the ability to chelate Fe2＋ and Cu2＋ and compared with carnosine and ascorbic acid. Totally 13 fractions were separated from the Taihe black-bone silky fowl muscle hydrolysate,and all of them had obvious antioxidant effect in a concentration-dependent way. Most of the fractions presented stronger abilities to scavenge hydroxyl free radicals,inhibit lipid peroxidation and chelate Fe2＋ than the hydrolysate.